Tumor Biology

, Volume 36, Issue 3, pp 1943–1953 | Cite as

Identification of OLIG2 as the most specific glioblastoma stem cell marker starting from comparative analysis of data from similar DNA chip microarray platforms

  • Anne-Laure Trépant
  • Christelle Bouchart
  • Sandrine Rorive
  • Sébastien Sauvage
  • Christine Decaestecker
  • Pieter Demetter
  • Isabelle Salmon
Research Article

Abstract

Despite advances in surgical and adjuvant treatments, overall survival of glioblastoma (GBM) patients remains poor. The cancer stem cell concept suggests that a rare stem cell population, called glioma stem cells (GSCs), has high ability to self-renewal leading to recurrence in GBM. The identification of specific markers of GSCs would provide a powerful tool to detect and to characterise them in order to develop targeted therapies. We carried out a comparative analysis based on the identification of inter-study concordances to identify the genes that exhibit at best differential levels of expression between GSC-enriched cell cultures and differentiated tumour cell cultures from independent studies using DNA chip microarray technologies. We finally studied the protein expression of the marker we considered the most specific by immunohistochemistry and semi-quantitative analysis on a retrospective series of 18 GBMs. Of the selected studies, 32 genes were retained. Among them, eight genes were identified to be overexpressed in GSC-enriched cultures compared to differentiated tumour cell cultures. Finally, among the eight genes, oligodendrocyte lineage transcription factor 2 (OLIG2) was characterised by the most different expression level in the “GSC model” compared to the “differentiated tumour cells model”. Our approach suggests that OLIG2 is the most specific GSC marker; additional investigations with careful considerations about methodology and strategies of validation are, however, mandatory.

Keywords

Olig2 Glioblastoma Glioma stem cells Microarray studies 

Notes

Acknowledgments

The authors thank A. Verrellen for excellent technical assistance.

Research support: This work was supported by a doctoral grant from the Fonds Erasme (ALT) and by the Fonds Yvonne Boël (Brussels, Belgium). The CMMI is supported by the European Regional Development Fund and Wallonia. C.D. is a Senior Research Associate with the FNRS.

Conflicts of interest

None

Supplementary material

13277_2014_2800_MOESM1_ESM.doc (108 kb)
ESM 1 (DOC 107 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Anne-Laure Trépant
    • 1
  • Christelle Bouchart
    • 2
  • Sandrine Rorive
    • 1
    • 3
  • Sébastien Sauvage
    • 3
  • Christine Decaestecker
    • 3
    • 4
  • Pieter Demetter
    • 1
  • Isabelle Salmon
    • 1
    • 3
  1. 1.Department of Pathology, Erasme University HospitalUniversité Libre de BruxellesBrusselsBelgium
  2. 2.Department of Radiotherapy-OncologyInstitut Jules BordetBrusselsBelgium
  3. 3.DIAPATH, Center of Microscopy and Molecular Imaging (CMMI)GosseliesBelgium
  4. 4.Laboratories of Image, Signal processing and Acoustics (LISA)Université Libre de BruxellesBrusselsBelgium

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