Tumor Biology

, Volume 36, Issue 3, pp 1701–1710 | Cite as

MicroRNA-93 suppress colorectal cancer development via Wnt/β-catenin pathway downregulating

  • Qingchao Tang
  • Zhaoxia Zou
  • Chendan Zou
  • Qian Zhang
  • Rui Huang
  • Xu Guan
  • Qiang Li
  • Zhongjing Han
  • Dayong Wang
  • Huiyan Wei
  • Xu Gao
  • Xishan Wang
Research Article

Abstract

MicroRNA-93 (miR-93) is involved in several carcinoma progressions. It has been reported that miR-93 acts as a promoter or suppressor in different tumors. However, till now, the role of miR-93 in colon cancer is unclear. Herein, we have found that expression of miR-93 was lower in human colon cancer tissue and colorectal carcinoma cell lines compared with normal colon mucosa. Forced expression of miR-93 in colon cancer cells inhibits colon cancer invasion, migration, and proliferation. Furthermore, miR-93 may downregulate the Wnt/β-catenin pathway, which was confirmed by measuring the expression level of the β-catenin, axin, c-Myc, and cyclin-D1 in this pathway. Mothers against decapentaplegic homolog 7 (Smad7), as an essential molecular protein for nuclear accumulation of β-catenin in the canonical Wnt signaling pathway, is predicted as a putative target gene of miR-93 by the silico method and demonstrated that it may be suppressed by targeting its 3′UTR. These findings showed that miR-93 suppresses colorectal cancer development via downregulating Wnt/β-catenin, at least in part, by targeting Smad7. This study revealed that miR-93 is an important negative regulator in colon cancer and suggested that miR-93 may serve as a novel therapeutic agent that offers benefits for colon cancer treatment.

Keywords

Colorectal cancer MicroRNA-93 Wnt/β-catenin Invasion Proliferation 

Notes

Funding

The work was supported by the National Natural Science Foundation of China (81272706).

Conflicts of interest

None.

Supplementary material

13277_2014_2771_MOESM1_ESM.doc (118 kb)
ESM 1 (DOC 118 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Qingchao Tang
    • 1
  • Zhaoxia Zou
    • 2
  • Chendan Zou
    • 2
  • Qian Zhang
    • 1
  • Rui Huang
    • 1
  • Xu Guan
    • 1
  • Qiang Li
    • 3
  • Zhongjing Han
    • 2
  • Dayong Wang
    • 2
  • Huiyan Wei
    • 2
  • Xu Gao
    • 2
  • Xishan Wang
    • 1
    • 4
  1. 1.Department of Colorectal Cancer Surgery, Cancer Center, the Second Affiliated HospitalHarbin Medical UniversityHarbinChina
  2. 2.Department of Biochemistry and Molecular BiologyHarbin Medical UniversityHarbinChina
  3. 3.Department of General Surgery, the Second Affiliated HospitalHarbin Medical UniversityHarbinChina
  4. 4.Colorectal Cancer Institute, the Second Affiliated HospitalHarbin Medical UniversityHarbinChina

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