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Tumor Biology

, Volume 36, Issue 2, pp 1221–1231 | Cite as

Identification of prohibitin 1 as a potential prognostic biomarker in human pancreatic carcinoma using modified aqueous two-phase partition system combined with 2D-MALDI-TOF-TOF-MS/MS

  • Ning Zhong
  • Yazhou Cui
  • Xiaoyan Zhou
  • Tianliang Li
  • Jinxiang Han
Research Article

Abstract

Membrane proteins are an important source of potential targets for anticancer drugs or biomarkers for early diagnosis. In this study, we used a modified aqueous two-phase partition system combined with two-dimensional (2D) matrix-assisted laser desorption ionization (MALDI) time of flight (TOF) mass spectrometry (MS, 2D-MALDI-TOF-TOF-MS/MS) analysis to isolate and identify membrane proteins in PANC-1 pancreatic cancer cells. Using this method, we identified 55 proteins, of which 31 (56.4 %) were membrane proteins, which, according to gene ontology annotation, are associated with various cellular processes including cell signal transduction, differentiation, and apoptosis. Immunohistochemical analysis showed that the expression level of one of the identified mitochondria membrane proteins, prohibitin 1 (PHB1), is correlated with pancreatic carcinoma differentiation; PHB1 is expressed at a higher level in normal pancreatic tissue than in well-differentiated carcinoma tissue. Further studies showed that PHB1 plays a proapoptotic role in human pancreatic cancer cells, which suggests that PHB1 has antitumorigenic properties. In conclusion, we have provided a modified method for isolating and identifying membrane proteins and demonstrated that PHB1 may be a promising biomarker for early diagnosis and therapy of pancreatic (and potentially other) cancers.

Keywords

Pancreatic carcinoma Prohibitin 1 Tumor biomarker Membrane proteome Apoptosis 

Notes

Acknowledgments

This study was supported by grants from the Technologies R&D Program of Shandong Province, China (2005GG1102003) and the National High Technology Research and Development Program of China (2007AA021004).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Ning Zhong
    • 1
  • Yazhou Cui
    • 2
  • Xiaoyan Zhou
    • 2
  • Tianliang Li
    • 2
  • Jinxiang Han
    • 1
    • 2
  1. 1.School of MedicineShandong UniversityJinanChina
  2. 2.Key Laboratory of Ministry of Health for Biotech-Drug, Shandong Medicinal Biotechnology CenterShandong Academy of Medical SciencesJinanChina

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