The SNAI1 3′UTR functions as a sponge for multiple migration-/invasion-related microRNAs
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Accumulating evidence has indicated a large-scale regulatory network generated by 3′untranslated regions (3′UTRs) in cancer. The 3′UTRs act not only in cis but, most likely even more importantly, as trans regulators of gene expression, consequently leading to phenotypic alterations. Here, we found that ectopic expression of SNAI1 3′UTR induced migration and invasion of ovarian cancer cell line RMUG-L without significantly affecting cell viability. Additionally, SNAI1 3′UTR overexpression regulated key epithelial-to-mesenchymal transition (EMT) markers, including SNAI1, Vimentin, and E-cadherin, and functioned as a sponge for multiple migration-/invasion-related microRNAs (miRNAs) in RMUG-L cells. These findings revealed the noncoding function of SNAI1 for the first time.
KeywordsOvarian cancer SNAI1 3′UTR Epithelial-to-mesenchymal transition MicroRNA Competing endogenous RNA
This work was supported by grants from the Natural Science Foundation of China (No.81172456), the Natural Science Foundation of Shanghai (No.124119a5502), and the Ministry of Education of the People’s Republic of China for Creative Ph.D. Students (No.JFF157001).
Conflicts of interest
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