Tumor Biology

, Volume 36, Issue 2, pp 1039–1044 | Cite as

Diagnostic value of serum M30 and M65 in patients with nasopharyngeal carcinoma

  • Fatma Sen
  • Ibrahim Yildiz
  • Hatice Odabas
  • Makbule Tambas
  • Leyla Kilic
  • Ahmet Karadeniz
  • Musa Altun
  • Meltem Ekenel
  • Murat Serilmez
  • Derya Duranyildiz
  • Sevil Bavbek
  • Mert Basaran
Research Article


M30 and M65 are circulating fragments of cytokeratin 18 released during apoptotic cell death and regarded as markers of cell death in patients with various tumor types. Our aim was to investigate the clinical and prognostic significance of the serum M30 and M65 concentrations in patients with advanced nasopharyngeal carcinoma. Thirty-two patients with nasopharyngeal cancer and 32 control subjects were investigated. Serum samples were obtained on first admission before any treatment was initiated. Serum M30 and M65 concentrations were measured by quantitative enzyme-linked immunosorbent assay. Median serum M30 (181.5 vs. 45.5 U/L, p < 0.001) and M65 (384.2 vs. 179.1 U/L, p < 0.001) concentrations were significantly higher in patients with advanced nasopharyngeal carcinomas than in controls. receiver operating characteristic (ROC) analysis showed that a cutoff for M30 of 225 U/L had a sensitivity of 62.5 % and a specificity of 73.9 % (area under the curve (AUC) = 0.592, 95 % confidence interval (CI) 35.3–83.2, p = 0.44), while a cutoff for M65 of 423.4 U/L had a sensitivity of 75.1 % and a specificity of 65.6 % (AUC = 0.562, 95 % CI 36.0–76.5, p = 0.60). However, serum M30 and M65 were not important prognostic factors for progression-free survival. There were no statistically significant correlations between serum M30 and M65 concentrations and clinicodemographical variables. Serum M30 and M65 concentrations were found to have a diagnostic value in nasopharyngeal cancer. However, neither M30 nor M65 serum levels played a prognostic role in the outcome in nasopharyngeal cancer patients.


Biomarker Cytokeratin 18 Diagnostic value Nasopharyngeal carcinoma Serum M30 level Serum M65 level 



This work has no funding source.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Fatma Sen
    • 1
  • Ibrahim Yildiz
    • 1
  • Hatice Odabas
    • 1
  • Makbule Tambas
    • 2
  • Leyla Kilic
    • 1
  • Ahmet Karadeniz
    • 2
  • Musa Altun
    • 2
  • Meltem Ekenel
    • 1
  • Murat Serilmez
    • 3
  • Derya Duranyildiz
    • 3
  • Sevil Bavbek
    • 1
  • Mert Basaran
    • 1
  1. 1.Department of Medical Oncology, Institute of OncologyIstanbul UniversityIstanbulTurkey
  2. 2.Department of Radiation Oncology, Istanbul Faculty of MedicineIstanbul UniversityIstanbulTurkey
  3. 3.Department of Basic Oncology, Institute of OncologyIstanbul UniversityIstanbulTurkey

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