Decreased expression of EFS is correlated with the advanced prostate cancer
- 221 Downloads
Prostate cancer is the most frequently diagnosed malignant neoplasm in men in the developed countries. Although the progression of prostate cancer and the processes of invasion and metastasis by tumor cells are comparatively well understood, the genes involved in these processes are not fully determined. Therefore, a common area of research interest is the identification of novel molecules that are involved in these processes. In the present study, we have used in silico and experimental approaches to compare the expression of embryonal Fyn-associated substrate (EFS) between normal prostate and prostate cancer. We showed that EFS expression is remarkably downregulated in prostate cancer cells, compared to normal prostate cells. We also found that decreased expression of EFS in prostate cancer cells is due to DNA methylation. In addition, we showed that high EFS expression is important to suppress a malignant behavior of prostate cancer cells. Therefore, we suggest that EFS should be considered as a novel tumor suppressor gene in prostate cancer.
KeywordsEFS Prostate cancer DNA methylation
Embryonal Fyn-associated substrate
Breast cancer resistance 1
Neural precursor cell expressed, developmentally downregulated 9
Fetal bovine serum
Tris-buffered saline containing 0.1 % Tween 20
Glyceraldehyde 3-phosphate dehydrogenase
We would like to thank all the members of Harran University Central Laboratory (HUMEL) for their help and assistance. We would also like to thank Prof. Dr. Kemal S. Korkmaz from Ege University, Department of Bioengineering, İzmir, Turkey, for providing PC-3, DU145, and LNCaP cell lines and Assoc. Prof. Dr. Omer Faruk Bayraktar from Yeditepe University, Medical Faculty, Department of Medical Biology, Istanbul, Turkey, for providing PNT1a cell line. This study was supported by grants 113S433 and 113S290 from the Scientific and Technological Research Council of Turkey (TUBITAK) and 13044 from Harran University Scientific Research Project Unit (HUBAK) to L.V.
Conflicts of interest
- 10.Law SF, Estojak J, Wang B, Mysliwiec T, Kruh G, Golemis EA. Human enhancer of filamentation 1, a novel p130cas-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in saccharomyces cerevisiae. Mol Cell Biol. 1996;16:3327–37.CrossRefPubMedPubMedCentralGoogle Scholar
- 20.Varisli L, Gonen-Korkmaz C, Syed HM, Bogurcu N, Debelec-Butuner B, Erbaykent-Tepedelen B, et al. Androgen regulated HN1 leads proteosomal degradation of androgen receptor (AR) and negatively influences ar mediated transactivation in prostate cells. Mol Cell Endocrinol. 2012;350:107–17.CrossRefPubMedGoogle Scholar