Tumor Biology

, Volume 36, Issue 2, pp 1105–1113 | Cite as

Cripto-1 expression and its prognostic value in human bladder cancer patients

  • Bingbing Wei
  • Wei Jin
  • Jun Ruan
  • Zhuoqun Xu
  • You Zhou
  • Jiabei Liang
  • Huan Cheng
  • Ke Jin
  • Xing Huang
  • Peng Lu
  • Qiang Hu
Research Article

Abstract

Cripto-1 is an important embryonic gene that involved in self-renewal and maintenance of pluripotency of stem cells. Overexpression of Cripto-1 has been found to be correlated with tumorigenesis and may affect tumor recurrence and metastasis. The previous studies indicate that Cripto-1 might be a potential prognostic biomarker for several malignancies. The aim of this study is to examine Cripto-1 expression pattern and clinicopathological significance in human bladder cancer patients. We investigated Cripto-1 expression in 30 paired bladder cancer tissues and corresponding noncancerous bladder tissues using real-time quantitative RT-PCR (qRT-PCR). Moreover, Cripto-1 expression in 130 bladder cancer specimens and bladder cancer T24 and 5637 cells were analyzed using immunohistochemistry and immunofluorescence assays. The recurrence/metastasis-free survival was assessed by Kaplan–Meier method and log-rank test. Cox regression was also used for univariate and multivariate analyses of prognostic factors. The results showed that Cripto-1 expression is increased in bladder cancer tissues and is significantly associated with tumor size (P = 0.005) and tumor grade (P = 0.035). In addition, the expression level of Cripto-1 in bladder cancer was also found to be significantly associated with SRY-related HMG-box gene 2 expression (P = 0.003) and Ki-67 (P = 0.001). Compared with the patients with low Cripto-1 expression, the patients with high Cripto-1 expression had significantly poorer recurrence/metastasis-free survival (P = 0.011). Cox regression showed that Cripto-1 might be an independent prognostic factor for recurrence/metastasis-free survival (P = 0.036). Our findings suggest that high Cripto-1 expression might be involved in the development of bladder cancer and a potentially effective prognostic marker in bladder cancer patients.

Keywords

Cripto-1 Bladder cancer Prognosis 

Notes

Acknowledgments

The work was supported by grants from the Key Program of Hospital Management Center, China (YGZ1105); Youth Foundation of Jiangsu Health Department, China (Q201207); Science and Technology Development Foundation of Wuxi, China (CSE01N1108); and Foundation of Nanjing Medical University, China (NJMU034).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Bingbing Wei
    • 1
  • Wei Jin
    • 2
  • Jun Ruan
    • 1
  • Zhuoqun Xu
    • 1
  • You Zhou
    • 3
  • Jiabei Liang
    • 4
  • Huan Cheng
    • 5
  • Ke Jin
    • 1
  • Xing Huang
    • 1
  • Peng Lu
    • 1
  • Qiang Hu
    • 1
  1. 1.Department of Urology, Affiliated Wuxi People’s HospitalNanjing Medical UniversityWuxiChina
  2. 2.Division of Human BiologyFred Hutchinson Cancer Research CenterSeattleUSA
  3. 3.Minerva Foundation Institute for Medical ResearchHelsinkiFinland
  4. 4.Department of Pathology, Affiliated Wuxi People’s HospitalNanjing Medical UniversityWuxiChina
  5. 5.Department of UrologyThe Affiliated Hospital of Xuzhou Medical CollegeXuzhouChina

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