Tumor Biology

, Volume 36, Issue 2, pp 1179–1190 | Cite as

Luteolin supplementation adjacent to aspirin treatment reduced dimethylhydrazine-induced experimental colon carcinogenesis in rats

  • Neamt H. A. Osman
  • Usama Z. Said
  • Ahmed M. El-Waseef
  • Esraa S. A. Ahmed
Research Article


Previous studies have shown that aspirin is used in colon cancer treatment. However, long-term of Aspirin usage is limited to gastric and renal toxicity. Luteolin (LUT) has cancer prevention and anti-inflammatory effects. The present study was designed to investigate the effect of LUT supplementation and Aspirin treatment in dimethylhydrazine (DMH)-induced carcinogenesis in rats. DMH (20 mg/kg BW/week) treated rats received gavages with Aspirin (50 mg/kg BW/week) and LUT (0.2 mg/kg BW/day) for 15 weeks. DMH injections induce colon polyps and renal bleeding, significantly increasing carcinoembryonic antigen (CEA), cyclooxygenase-2 (COX-2), oxidative stress, and kidney function tests and reducing antioxidant markers. Either Aspirin or LUT gavages alone or combined produce a significant decrease in colon polyp number and size, significantly decreasing CEA, COX-2, and oxidative stress and increasing antioxidant markers. In conclusion, the supplementations of LUT adjacent to Aspirin in the treatment of DMH-induced carcinogenesis in rats reflect a better effect than the use of Aspirin alone.


Dimethylhydrazine Aspirin Luteolin (LUT) Colon 


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Neamt H. A. Osman
    • 1
  • Usama Z. Said
    • 1
  • Ahmed M. El-Waseef
    • 2
  • Esraa S. A. Ahmed
    • 1
  1. 1.Radiation Biology Department, National Centre for Radiation Research and TechnologyAtomic Energy AuthorityNasr CityEgypt
  2. 2.College of ScienceEl Mansoura UniversityMansouraEgypt

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