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Comparative proteomic analysis of fibrosarcoma and skin fibroblast cell lines

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Tumor Biology

Abstract

Comparative proteomic analysis of normal and cancer cell lines provides for a better understanding of the molecular mechanism of cancer development and is essential for developing more effective strategies for new biomarker or drug target discovery. The purpose of this study is to compare protein expression levels between fibrosarcoma and fibroblast cell lines. In our study, two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) techniques were carried out to compare the protein profile between fibrosarcoma and fibroblast cell lines. We prepared cell lysate samples to analyze intracellular proteins and secretome samples to analyze extracellular proteins in both cell lines. Our results revealed 13 upregulated proteins and 1 downregulated protein of which all of them identified in fibrosarcoma cell line after the comparison with fibroblast cell line cell lysates. When comparing secretome profiles of both cell lines, we found and identified 13 proteins only expressed in fibrosarcoma cell line. These identified proteins have common functions such as cell proliferation, cell differentiation, invasion, metastasis, and apoptosis in cancer. The data obtained from this study indicates that these proteins have importance on understanding the molecular mechanism of fibrosarcoma. These proteins may serve as candidate biomarkers and drug targets for future clinical studies.

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References

  1. Fletcher CDM, Krishnan Unni K, Mertens F. Pathology and genetics of tumours of soft tissue and bone. 3rd ed. Lyon: IARC; 2006.

    Google Scholar 

  2. Nikitovic D, Kouvidi K, Karamanos NK, Tzanakakis GN. The roles of hyaluronan/RHAMM/CD44 and their respective interactions along the insidious pathways of fibrosarcoma progression. Biomed Res Int. 2013;2013:929531.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Hajdu SI. Fibrosarcoma: a historic commentary. Cancer. 1998;82:2081–9.

    Article  CAS  PubMed  Google Scholar 

  4. Goldblum JR, Folpe AL, Weiss SW. Enzinger and Weiss’s soft tissue tumors. 6th ed. Philadelphia: Elsevier Saunders; 2013.

    Google Scholar 

  5. Song B, Kim B, Choi SH, Song KY, Chung YG, Lee YS, et al. Mesenchymal stromal cells promote tumor progression in fibrosarcoma and gastric cancer cells. Korean J Pathol. 2014;48(3):217–24.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Klijanienko J, Lagace R. Soft tissue tumors: a multidisciplinary, decisional diagnostic approach. 1st ed. Hoboken: Wiley; 2011.

    Book  Google Scholar 

  7. Xue H, Lu B, Lai M. The cancer secretome: a reservoir of biomarkers. J Transl Med. 2008;6:52.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Zwickl H, Traxler E, Staettner S, Parzefall W, Grasl-Kraupp B, Karner J, et al. A novel technique to specifically analyze the secretome of cells and tissues. Electrophoresis. 2005;26:2779–85.

    Article  CAS  PubMed  Google Scholar 

  9. Guoqing L, Zhefeng X, Jianping L, Cui L, Feng L, Zhuchu C. Cancer: a proteomic disease. Sci China Life Sci. 2011;54:403–8.

    Google Scholar 

  10. Rubporn A, Srisomsap C, Subhasitanont P, Chokchamnankit D, Chiablaem K, Svasti J, et al. Comparative proteomic analysis of lung cancer cell line and lung fibroblast cell line. Cancer Genomics Proteomics. 2009;6:229–37.

    CAS  PubMed  Google Scholar 

  11. Zang J, Wang P, Gao S, Xiao D, Zhang J, Wang K. Differential proteins expression between gastric cancer and normal cell lines. Life Sci J. 2008;5(4):28–32.

    CAS  Google Scholar 

  12. Deng SS, Xing TY, Zhou HY, Xiong RH, Lu YG, Wen B, et al. Comparative proteome analysis of breast cancer and adjacent normal breast tissues in human. Genomics Proteomics Bioinforma. 2006;4(3):165–72.

    Article  CAS  Google Scholar 

  13. Johansson B, Pourian MR, Chuan Y, Byman I, Bergh A, Pang ST, et al. Proteomic comparison of prostate cancer cell lines LNCaP-FGC and LNCaP-r reveals heatshock protein 60 as a marker for prostate malignancy. Prostate. 2006;66:1235–44.

    Article  CAS  PubMed  Google Scholar 

  14. Antharavally BS, Mallia KA, Rangaraj P, Haney P, Bell PA. Quantitation of proteins using a dye-metal-based colorimetric protein assay. Anal Biochem. 2009;385(2):342–5.

    Article  CAS  PubMed  Google Scholar 

  15. Shen H, Huang J, Pei H, Zeng S, Tao Y, Shen L, et al. Comparative proteomic study for profiling differentially expressed proteins between chinese left and right sided colon cancers. Cancer Sci. 2013;104(1):135–41.

    Article  CAS  PubMed  Google Scholar 

  16. Yang MS, Wang HS, Wang BS, Li WH, Pang ZF, Zou BK, et al. A comparative proteomic study identified calreticulin and prohibitin up-regulated in adenocortical carcinomas. Diagn Pathol. 2013;8:58.

    PubMed  PubMed Central  Google Scholar 

  17. Coghlin C, Carpenter B, Dundas SR, Lawrie LC, Telfer C, Murray GI. Characterization and over-expression of chaperonin t-complex proteins in colorectal cancer. J Pathol. 2006;210:351–57.

    Article  CAS  PubMed  Google Scholar 

  18. Li C, Chen Z, Xiao Z, Wu X, Zhan X, Zhang X, et al. Comparative proteomic analysis of human lung squamous carcinoma. Biochem Biophys Res Commun. 2003;309:253–60.

    Article  CAS  PubMed  Google Scholar 

  19. Wang TH, Chao A, Tsai CL, Chang CL, Chen SH, Lee YS, et al. Stress-induced phosphoprotein 1 as a secreted biomarker for human ovarian cancer promotes cancer cell proliferation. Mol Cell Proteomics. 2010;9(9):1873–84.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Chang HY, Hor SY, Lim KP, Zain RB, Cheong SC, Rahman MA, et al. Oral cancer secretome: identification of cancer associated proteins. Electrophoresis. 2013;34(15):2199–208.

    Article  CAS  PubMed  Google Scholar 

  21. Dos Santos M, Da Cunha Mercante AM, Nunes FD, Leopoldino AM, De Carvalho MB, Gazito D, et al. Prognostic significance of ndrg1 expression in oral and oropharyngeal squamous cell carcinoma. Mol Biol Rep. 2012;39(12):10157–65.

    Article  PubMed  Google Scholar 

  22. Pino I, Pio R, Toledo G, Zabalegui N, Vicent S, Rey N, et al. Altered patterns of expression of members of the heterogeneous nuclear ribonucleoprotein (hnRNP) family in lung cancer. Lung Cancer. 2003;41(2):131–43.

    Article  PubMed  Google Scholar 

  23. Chaker S, Kashat L, Voisin S, Kaur J, Kak I, Macmillan C, et al. Secretome proteins as candidate biomarkers for aggressive thyroid carcinomas. Proteomics. 2013;13(5):771–87.

    Article  CAS  PubMed  Google Scholar 

  24. Ito K, Stannard K, Gabutero E, Clark AM, Neo SY, Onturk S, et al. Galectin-1 as a potent target for cancer therapy: role in the tumor microenvironment. Cancer Metastasis Rev. 2012;31:763–78.

    Article  CAS  PubMed  Google Scholar 

  25. Yin J, Chen G, Liu Y, Liu S, Wang P, Wan Y, et al. Downregulation of SPARC expression decreases gastric cancer cellular invasion and survival. J Exp Clin Cancer Res. 2010;29:59.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Kaneko T, Konno H, Baba M, Tanaka T, Nakamura S. Urokinase-type plasminogen activator expression correlates with tumor angiogenesis and poor outcome in gastric cancer. Cancer Sci. 2003;94(1):43–9.

    Article  CAS  PubMed  Google Scholar 

  27. Ge S, Mao Y, Yi Y, Xie D, Chen Z, Xiao Z. Comparative proteomic analysis of secreted proteins from nasopharyngeal carcinoma-associated stromal fibroblasts and normal fibroblast. Exp Ther Med. 2012;3:857–60.

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Sardana G, Marshall J, Diamandis EP. Discovery of candidate tumor markers for prostate cancer via proteomic analysis of cell culture conditioned medium. Clin Chem. 2007;53(3):429–37.

    Article  CAS  PubMed  Google Scholar 

  29. Sasaki T, Hankins GR, Helm GA. Major vault protein/lung resistance-related protein (MVP/LRP) expression in nervous system tumors. Brain Tumor Pathol. 2002;19(2):59–62.

    Article  PubMed  Google Scholar 

  30. Diaz-Ramos A, Roig-Borrellas A, Garcia-Melero A, Lopez-Alemany R. α-Enolase, a multifunctional protein: its role on pathophysiological situations. J Biomed Biotechnol. 2012;2012:156795.

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

The authors thank Dr. Tarik Baykal from Medipol University for the assistance with LC-MS/MS analysis and Dr. Mert Pekcan from Ankara University for providing technical assistance and suggestions. This study was supported by Ankara University Scientific Research Grant (grant no. 12B3338001).

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  1. Faculty of Veterinary Medicine, Department of Biochemistry, Ankara University, 06110, Ankara, Turkey

    Ogunc Meral & Hamdi Uysal

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  1. Ogunc Meral
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  2. Hamdi Uysal
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Correspondence to Hamdi Uysal.

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Meral, O., Uysal, H. Comparative proteomic analysis of fibrosarcoma and skin fibroblast cell lines. Tumor Biol. 36, 561–567 (2015). https://doi.org/10.1007/s13277-014-2672-8

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  • DOI: https://doi.org/10.1007/s13277-014-2672-8

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