RETRACTED ARTICLE: Diagnostic procedures for improving of the KIT (CD117) expressed allele burden for the liver metastases from uterus mast cell tumors: prognostic value of the metastatic pattern and tumor biology
- 821 Downloads
The activating KIT marker plays a central role in the pathogenesis, diagnosis, and targeted treatment of systemic mastocytosis (SM). Recent studies have identified the KIT (CD117) as a marker that distinguishes nonneoplastic from neoplastic mast cells in human systemic mastocytosis. In this study, we conclude that immunohistopathology assays for KIT staining pattern are useful complimentary tools for diagnosis and evaluation of prognosis in uterus mast cell tumor (MCT) metastasis to the liver in 10 patients. Uterine and hepatic cytology revealed mast cell neoplasia, which was confirmed as visceral mast cell tumor on postmortem examination. Histological changes of densely packed, poorly differentiated neoplastic mast cells, sheets of neoplastic round to pleomorphic cells that formed nonencapsulated nodules, high mitotic figures, necrosis, and fibrosis were found. In addition, eosinophils were scattered among the mast cells at the periphery of the nodules. These findings indicate tumors of high-grade malignancy with infiltrative cells resembling the uterus MCT in the intraparenchymal and periparenchymal areas of the liver. Immunohistochemically, tumors were positive for KIT. The histopathologic features coupled with the KIT immunoreactivity led to diagnosis of high-grade uterus MCTs. Taken together, these findings suggest that CD117 may play a critical role in early uterus MCT development and may be a stimulatory factor in grade 3 MCT. Therefore, the result has supported our hypothesis that there was an increased opportunity to observe a higher CD117 staining pattern in high-grade MCTs.
KeywordsImmunohistochemistry Histopathology KIT Neoplasia Uterine Liver
The authors thank Dr. Javad Javanbakht for his help with this manuscript.
Conflicts of interest
- 8.Yamamoto K. Electron microscopy of mast cells in the venous wall of canine liver. J Vet Med Sci. 2000;62:118.Google Scholar
- 19.Brodey RS, Roszel JF. Neoplasms of the canine uterus, vagina and vulva: a clinicopathologic survey of 90 cases. J Am Anim Hosp Assoc. 1967;151:1294–307.Google Scholar
- 20.Cotchin E. Neoplasia in the dog. Vet Rec. 1954;66:879–88.Google Scholar
- 21.Baldwin CJ, Roszel JF, Clark TP. Uterine adenocarcinoma in dogs. Compend Contin Educ Pract Vet. 1992;14:731–7.Google Scholar
- 25.Sailasuta A, Ketpun D, Piyaviriyakul P, Theerawatanasirikul S, Theewasutrakul P, Rungsipipat A: The Relevance of CD117-immunocytochemistry staining patterns to mutational exon-11 in c-kit detected by PCR from fine-needle aspirated canine mast cell tumor cells. Vet Med Int. 2014;2014:787498, doi: 10.1155/2014/787498.
- 35.Crafts GA, Pulley LT. Generalized cutaneous mast cell tumor in a cat. Feline Pract. 1975;5:57–8.Google Scholar
- 37.Withrow SJ, Vail DM: Withrow & MacEwen’s small animal clinical oncology.5th ed. St Louis, United States: Saunders Elsevier, 2013, 20:335–55.Google Scholar
- 49.Gross TL, Ihrke PJ, Walder EJ, Affolter VR: Epithelial neoplasms and other tumors. 2nd edition. Blackwell Publishing: Skin diseases of the dog and cat: clinical and histopathologic diagnosis; 2005:641–54.Google Scholar
- 50.Bronden LB, Eriksen T, Kristensen AT. Mast cell tumours and other skin neoplasia in Danish dogs—data from the Danish Veterinary Cancer Registry. Acta Vet Scand. 2010;22:52–6.Google Scholar
- 52.Javanbakht J, Pedram B, Taheriyan MR, Khadivar F, Hosseini SH, Abdi FS, et al. Canine transmissible venereal tumor and seminoma: a cytohistopathology and chemotherapy study of tumors in the growth phase and during regression after chemotherapy. Tumour Biol. 2014;35(6):5493–500.CrossRefPubMedGoogle Scholar
- 53.Manesh JY, Shafiee R, Pedram B, Malayeri HZ, Mohajer S, Ahmadi S, Ahmadi S, Javanbakht J, Mokarizadeh A, Khadivar F. Improving the diagnosis, treatment, and biology patterns of feline mammary intraepithelial lesions: a potential model for human breast masses with evidence from epidemiologic and cytohistopathologic studies. Tumour Biol 2014 Aug 21.Google Scholar