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Tumor Biology

, Volume 36, Issue 1, pp 213–217 | Cite as

Hsp27 (HSPB1) differential expression in normal salivary glands and pleomorphic adenomas and association with an increased Bcl2/Bax ratio

  • Elisa C. de Siqueira
  • Fabrício T. A. Souza
  • Marina G. Diniz
  • Ricardo S. Gomez
  • Carolina C. Gomes
Research Article

Abstract

Pleomorphic adenoma (PA) is the most common salivary gland neoplasm. The Hsp27 (HSPB1) is an antiapoptotic protein whose synthesis follows cytotoxic stresses and result in a transient increase in tolerance to subsequent cell injury. Although Hsp27 is expressed in a range of normal tissues and neoplasms, a wide variation in its expression exists among different cells and tissues types. In certain tumours of glandular origin (such as oesophageal adenocarcinomas), the level of Hsp27 is decreased. In the present study, Hsp27 protein levels were evaluated by enzyme-linked immunosorbent assay (ELISA) in a set of 18 fresh PA and 12 normal salivary gland samples. In addition, we tested if Hsp27 protein levels correlated with p53 expression and cell proliferation index, as well as with the transcriptional levels of Bcl-2-associated X protein (BAX), B cell lymphoma 2 (BCL2) and Caspase 3 in PA. We further tested the association between Hsp27 expression and PA tumour size. While all normal salivary gland samples expressed Hsp27 protein, only half of the PA samples expressed it, resulting in a reduced expression of Hsp27 in PA when compared with normal salivary glands (P = 0.003). The expression levels of this protein correlated positively with a higher messenger ribonucleic acid (mRNA) ratio of Bcl2/Bax (R = 0.631; P = 0.01). In conclusion, a decreased Hsp27 protein expression level in PA was found. In addition, Hsp27 levels correlated positively with the Bcl2/Bax mRNA ratio, suggesting an antiapoptotic effect.

Keywords

Salivary gland tumours hsp Heat shock protein Apoptosis Pleomorphic adenoma p53 

Abbreviations

Bax

Bcl-2-associated X protein

Bcl2

B cell lymphoma 2

CASP3

Caspase 3

DNA

Deoxyribonucleic acid

ELISA

Enzyme-linked immunosorbent assay

Hsp

Heat shock protein

IHC

Immunohistochemistry

PA

Pleomorphic adenoma

PCR

Polymerase chain reaction

RNA

Ribonucleic acid

Notes

Acknowledgments

This work was supported by grants from the National Council for Scientific and Technological Development (CNPq)/Brazil, Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)/Brazil. Gomez R.S. and Gomes C.C. are research fellows at CNPq. Diniz M.G. is research fellow at Coordination for the Improvement of Higher Education Personnel (CAPES)/Brazil

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Elisa C. de Siqueira
    • 1
  • Fabrício T. A. Souza
    • 1
  • Marina G. Diniz
    • 1
  • Ricardo S. Gomez
    • 1
  • Carolina C. Gomes
    • 2
  1. 1.Department of Oral Pathology and Medicine, School of DentistryUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  2. 2.Department of Pathology, Biological Sciences InstituteUniversidade Federal de Minas GeraisBelo HorizonteBrazil

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