Tumor Biology

, Volume 36, Issue 1, pp 437–446 | Cite as

The expression profiles of microRNAs in Kaposi’s sarcoma

  • Xiu-Juan Wu
  • Xiong-Ming Pu
  • Zong-Feng Zhao
  • Ya-Nan Zhao
  • Xiao-Jing Kang
  • Wei-Dong Wu
  • Yun-Min Zou
  • Cao-Ying Wu
  • Yuan-Yuan Qu
  • De-Zhi Zhang
  • Yan-Yan Feng
  • Jian-Yong Liu
Research Article

Abstract

Kaposi’s sarcoma (KS) is a multicentric angioproliferative tumor of mesenchymal origin. The molecular and biologic aspects of KS are not fully understood. MicroRNAs are non-protein-coding small RNAs in the size range 19–25 nucleotides (nt) that play important roles in biological processes, including cellular differentiation, proliferation, and death. We performed a miRNA microarray analysis by detecting six paired KS and matched adjacent healthy tissues using the 7th generation of miRCURYTM LNA Array (v.18.0) (Exiqon) containing 3100 capture probes. We selected 10 significant differentially expressed miRNAs, which were confirmed by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) in 18 paired KS and matched adjacent healthy tissue specimens. We also investigated the associations between clinical features and miRNA expression. Among the 3100 human miRNA probes in the microarrays, we identified 170 differentially expressed miRNAs (69 upregulated and 101 downregulated miRNAs) in KS versus adjacent healthy tissues. Among the most significantly upregulated miRNAs were miR-126-3p, miR-199a-3p, miR-16-5p, and the 13 KSHV-related miRNAs. The most significantly downregulated miRNAs included miR-125b-1-3p and miR-1183. Eight upregulated miRNAs, miR-181b-5p, miR-199a-3p, miR-15a-5p, miR-126-3p, miR-1297, kshv-miR-k12-12-3p, kshv-miR-k12-1-5p, and miR-16-5p, and two downregulated miRNAs, miR-125b-1-3p and miR-1183, were confirmed by qRT-PCR in 18 paired KS samples. The qRT-PCR results for 10 miRNAs were consistent with our microarray results. The miR-125b-1-3p and miR-16-5p had statistically significant associations with HHV-8 and HIV infections in KS. The results of miRNA profiling showed that KS appears to have unique expression patterns when compared with paired adjacent healthy tissues, suggesting that deregulation of miRNAs plays an important role in the progression of KS. These differentially expressed miRNAs may provide novel diagnostic and prognostic tools.

Keywords

MicroRNA Profile Expression Kaposi’s sarcoma 

Notes

Acknowledgments

This work was supported by a grant from the Natural Science Foundation of China (Grant No. 81260311) and Natural Science Foundation of Xinjiang Uygur Autonomous Region (Grant No. 2014211A059). We are most grateful to all the families who have so willingly participated in this study and made this study possible. We also acknowledge the support of the Clinical Dermatology Institute of Xinjiang Uygur Autonomous Region and National Clinical key specialty construction projects.

Conflicts of interest

None

Supplementary material

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Supplementary Table 1 (DOCX 14 kb)
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Supplementary Figure 3 (DOCX 238 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Xiu-Juan Wu
    • 1
    • 2
  • Xiong-Ming Pu
    • 1
    • 2
  • Zong-Feng Zhao
    • 3
  • Ya-Nan Zhao
    • 2
  • Xiao-Jing Kang
    • 2
  • Wei-Dong Wu
    • 3
  • Yun-Min Zou
    • 2
  • Cao-Ying Wu
    • 2
  • Yuan-Yuan Qu
    • 2
  • De-Zhi Zhang
    • 2
  • Yan-Yan Feng
    • 2
  • Jian-Yong Liu
    • 2
  1. 1.Department of Dermatology and VenereologyAnhui Medical UniversityHefeiChina
  2. 2.Department of Dermatology and VenereologyPeople’s Hospital of Xinjiang, Uygur Autonomous RegionUrumqiChina
  3. 3.Clinical Medical Research CenterPeople’s Hospital of Xinjiang, Uygur Autonomous RegionUrumqiChina

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