Tumor Biology

, Volume 35, Issue 12, pp 12535–12543 | Cite as

Increased expression levels of S100A4 associated with hypoxia-induced invasion and metastasis in esophageal squamous cell cancer

  • Xiaoyan Xuan
  • Qianru Li
  • Zhongning Zhang
  • Ying Du
  • Pingping Liu
Research Article


Here, we explored the expression of S100A4 in esophageal squamous cell cancer (ESCC) tissues and investigated its role in hypoxia-induced invasion and metastasis in ESCC cell lines EC-1 and EC-9706. Immunohistochemistry analysis demonstrated that S100A4 was overexpressed in human ESCC tissues especially in ESCC tissues with deep invasion and lymph node metastasis. Hypoxia-induced S100A4 overexpression was observed in EC-1 and EC-9706 cells, in which it was associated with invasion and metastasis. Furthermore, we used EC-1 and EC-9706 cells again to upregulate or knockdown the expression S100A4 to investigate the mechanism role of S100A4 in hypoxia-induced invasion and metastasis in ESCC cells. And the results showed that S100A4 played an important role in promoting the invasion and metastasis of EC-1 and EC-9706 cells under hypoxia. Therefore, S100A4 overexpression might be an important mechanism by which hypoxia induced invasion and metastasis, and S100A4 could also be a potential target for the treatment of ESCC.


Esophageal cancer S100A4 Hypoxia Invasion and metastasis 



We thank Prof. Li Shanshan and Dr. Wang Feng for retrieving the tumor specimens and performing the H&E staining and Prof. Du Xiantang for proofreading the manuscript.

Grant support

This work was supported by grants from the funding scheme for young teachers of Zhengzhou University.


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Xiaoyan Xuan
    • 1
  • Qianru Li
    • 1
  • Zhongning Zhang
    • 2
  • Ying Du
    • 1
  • Pingping Liu
    • 1
  1. 1.Department of Microbiology and ImmunologyZhengzhou UniversityZhengzhouChina
  2. 2.The Second Affiliated Hospital of Zhengzhou UniversityZhengzhouChina

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