Tumor Biology

, Volume 36, Issue 3, pp 1455–1462 | Cite as

PCAF-mediated Akt1 acetylation enhances the proliferation of human glioblastoma cells

Research Article

Abstract

Glioblastoma is the most aggressive malignant primary brain tumor in humans. The activation of PI3K/Akt1 signaling pathway is involved in the proliferation of glioblastoma; however, the underlying mechanism of Akt1 activation during the development of glioblastoma remains largely unclear. Recently, the modification of molecular molecules at protein level such as acetylation has been shown to be related to the function of these molecules. Thus, in our present studies, the acetylation of Akt1 molecule and its role in the proliferation of glioblastoma cells was explored. The results showed that Akt1 was markedly acetylated in glioblastoma cells compared to normal human astrocytes. Mechanistically, PCAF-mediated Akt1 acetylation enhanced Akt1 phosphorylation at both sites of Thr308 and Ser473 and further promoted the proliferation of glioblastoma cells. Together, these data implicate that, as a post-translational regulation, PCAF-mediated Akt1 acetylation plays an important role in the proliferation of human glioblastoma, suggesting a novel target for clinical application.

Keywords

Glioblastoma Proliferation Akt1 Acetylation PCAF 

Abbreviations

CCK-8

Cell counting kit-8

shRNA

Short hairpin RNA

WCE

Whole-cell extract

IB

Immunoblot

co-IP

Co-immunoprecipitation

OD

Optical density

PCAF

P300/CBP-associated factor

HOXA10

Homeobox A10

DMEM

Dulbecco’s modified Eagle’s medium

FBS

Fetal bovine serum

PBS

Phosphate-buffered saline

HAT

Histone acetyltransferases

Notes

Acknowledgments

This work was supported by the China Natural Science Foundation (81000963, 81370062, and 81302196), Jiangsu Province’s 333 Talent Program (BRA2011046), Jiangsu Province “six personnel peak” funded projects (2013-WSN-028), Jiangsu Province’s Natural Science Foundation (BK2012670), Medical Research Foundation by Jiangsu Province Health Department (YG201301 and Z201318), the Clinical Technology Development of Jiangsu University (JLY20120053), the Kunshan Social Development Foundation (KS1006, KS1009), and the Suzhou Social Development Foundation (SYS201063).

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  1. 1.Department of Neurosurgery, The First People’s Hospital of KunshanJiangsu UniversitySuzhouPeople’s Republic of China
  2. 2.Department of NeurosurgeryFourth Affiliated Yancheng Hospital of Nantong UniversityYanchengPeople’s Republic of China
  3. 3.Department of Neurosurgery, Suzhou Kowloon HospitalShanghai Jiao Tong University School of MedicineSuzhouPeople’s Republic of China

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