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Tumor Biology

, Volume 35, Issue 12, pp 12157–12163 | Cite as

MicroRNA-191 promotes pancreatic cancer progression by targeting USP10

  • Hua Liu
  • Xuan-Fu Xu
  • Yan Zhao
  • Mao-Chun Tang
  • Ying-Qun Zhou
  • Jie Lu
  • Feng-Hou Gao
Research Article

Abstract

Recent studies have shown that microRNAs, a class of small and noncoding RNA molecules, play crucial roles in the initiation and progression of pancreatic cancer. In the present study, the expression and roles of miR-191 were investigated. Through both gain-of function and loss-of function experiments, a pro-oncogenic function of miR-191 was demonstrated. At the molecular level, bioinformatic prediction, luciferase, and protein expression analysis suggested that miR-191 could inhibit protein levels of UPS10, which suppressed the proliferation and growth of cancer cells through stabilizing P53 protein. Collectively, these data suggest that miR-191 could promote pancreatic cancer progression through targeting USP10, implicating a novel mechanism for the tumorigenesis.

Keywords

Pancreatic cancer MicroRNA miR-191 UPS10 P53 Gene regulation 

Notes

Conflicts of interest

None

Supplementary material

13277_2014_2521_Fig8_ESM.gif (7 kb)
Supplementary Fig. 1

a Relative expression levels of miR-191 after transfection of miR-191 mimics or negative controls (NC) in SW-1990 cells for 36 h. b, c The cell viability (CCK-8 assays, b) and proliferative potential (BrdU assays, c) was determined in SW-1990 cells transfected with miR-191 mimics or NC. d The cell-cycle phase of SW-1990 cells transfected with miR-191 mimics or NC was analyzed by flow cytometry. e, f Cell migration and invasion abilities were determined in SW-1990 cells. After transfected with miR-191 mimics or NC for 24 h, the SW-1990 cells were seeded in Transwell filters (e) or ECM gel-coated Transwell culture chambers (f) and incubated for 24 h, then the Transwell migration assay (e) or invasion assay (f) were conducted. *P < 0.05; **P < 0.01; ***P < 0.001 compared with NC. (GIF 7 kb)

13277_2014_2521_MOESM1_ESM.tif (600 kb)
High-resolution image (TIFF 600 kb)
13277_2014_2521_Fig9_ESM.gif (5 kb)
Supplementary Fig. 2

a, b The cell viability (CCK-8 assays, a) and proliferative potential (BrdU assays, b) was determined in SW-1990 cells transfected with miR-191 antisense (AS) or negative control (NC). c, d Transwell migration (c) or invasion assays (d) were conducted in SW-1990 cells transfected with miR-191 AS or NC. *P < 0.05; **P < 0.01; ***P < 0.001 compared with NC. (GIF 4 kb)

13277_2014_2521_MOESM2_ESM.tif (371 kb)
High-resolution image (TIFF 371 kb)
13277_2014_2521_Fig10_ESM.gif (2 kb)
Supplementary Fig. 3

a, b Protein levels of USP10 were determined by western blot in SW-1990 cells transfected with miR-191 mimics (a), antisense (b), or negative control (NC). (GIF 2 kb)

13277_2014_2521_MOESM3_ESM.tif (145 kb)
High-resolution image (TIFF 144 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  1. 1.Department of GastroenterologyThe Tenth Hospital Affiliated to Tongji UniversityShanghaiChina
  2. 2.Central LaboratoryThe 3rd Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghaiChina

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