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Tumor Biology

, Volume 35, Issue 11, pp 11229–11235 | Cite as

Vascular marker expression during the development of various types of gynaecological malignancy

  • Peter Urban
  • Miroslava Bilecova Rabajdova
  • Jan Varga
  • Peter Kruzliak
  • Viera Fialkovicova
  • Eva Durovcova
  • Maria Marekova
Research Article

Abstract

Clinical diagnosis of gynaecological malignancies is usually successful in the advanced stages of the tumour, and this has a major impact on the success of therapy. Therefore, in the last few years, cancer research has tried to identify and characterise new biochemical and molecular markers needed as predictive indicators for the diagnosis of cancer. Our aim has been to search the molecular changes in gene expression of death receptor 6, glycoprotein M6B (Gpm6B) and genes associated with tumours of the female genital system. After isolation of messenger RNA (mRNA), transcription of mRNA into the cDNA was performed. The quantification of gene expression changes was detected using the reverse transcription polymerase chain reaction (RT-PCR) method. Analysis at the protein level was performed using the Western blot method. In both methods, we used actin as a housekeeping gene for normalisation. Numerical quantification of changes in expression and in the level of the specific proteins was evaluated using the Data Syngene program. Significant changes in the levels of protein and mRNA expression were detected, mainly in the death receptor 6 (Dr6) gene of patients suffering from cancer of the corpus and cervix uteri and ovarian cancer, which also corresponded with the level of protein Dr6. At the level of transcription, a significant increase in the expression levels of mRNA for the Gpm6B gene was detected, which led to an increase in corresponding protein in the peripheral blood of patients with gynaecological tumours against the healthy control group. This article could help to find an adequate marker for clinical application that will enable more sensitive detection of the early stages of gynaecological malignancies from the peripheral blood of patients.

Keywords

Ovarian cancer Gene expression Vascular markers Death receptor 

Notes

Acknowledgments

This work was supported by the grant of the European Regional Development Fund—Project FNUSA-ICRC (No. CZ.1.05/1.1.00/02.0123), DIAGONKO ITMS: 26220220153 and the grant project VEGA 1/0115/14 from the Ministry of Education, Science, Research and Sport of the Slovak Republic.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Peter Urban
    • 1
  • Miroslava Bilecova Rabajdova
    • 1
  • Jan Varga
    • 2
  • Peter Kruzliak
    • 3
  • Viera Fialkovicova
    • 2
  • Eva Durovcova
    • 1
  • Maria Marekova
    • 1
  1. 1.Department of Medical and Clinical Biochemistry, Faculty of MedicinePavol Jozef Safarik UniversityKosiceSlovak Republic
  2. 2.2nd Department of Gynaecology and Obstetrics, Faculty of MedicinePavol Jozef Safarik UniversityKosiceSlovak Republic
  3. 3.International Clinical Research CenterSt. Anne’s University Hospital and Masaryk UniversityBrnoCzech Republic

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