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Tumor Biology

, Volume 35, Issue 11, pp 10897–10904 | Cite as

MicroRNA-124 inhibits cellular proliferation and invasion by targeting Ets-1 in breast cancer

  • Wentao Li
  • Wenqiao Zang
  • Pei Liu
  • Yuanyuan Wang
  • Yuwen Du
  • Xiaonan Chen
  • Meng Deng
  • Wencong Sun
  • Lei Wang
  • Guoqiang Zhao
  • Baoping Zhai
Research Article

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that, by targeting certain messenger RNAs (mRNAs) for translational repression or cleavage, can regulate the expression of these genes. In addition, miRNAs may also function as oncogenes and tumor-suppressor genes, as the abnormal expression of miRNAs is associated with various human tumors. However, the effects of the expression of miR-124 in breast cancer remain unclear. The present study was conducted to study the expression of miR-124 in breast cancer, paying particular attention to miR-124’s relation to the proliferation, invasion, and apoptosis in breast cancer cell MCF-7 and MDA-MB-231. Real-time quantitative RT-PCR (qRT-PCR) was performed to identify miR-124 that was down-regulated in breast cancer tissues. We also showed E26 transformation specific-1 (Ets-1) and miR-124 expression levels in breast cancer tissues that were associated with lymph node metastases. With transfected synthetic miR-124 agomir into MCF-7 and MDA-MB-231, a significant reduction (P < 0.05) in MCF-7 and MDA-MB-231 cell proliferation and colony forming potential was observed after treatment with miR-124. Apoptosis and migration rates were found to be significantly higher in two breast-derived cell lines transfected with a miR-124 agomir (P < 0.05). Luciferase reporter assay and Western blot were used to verify Ets-1 as a potential major target gene of miR-124, and the result showed that miR-124 can bind to putative binding sites within the Ets-1 mRNA 3′ untranslated region (UTR) to reduce its expression. Based on these findings, we propose that miR-124 and Ets-1 may serve as a therapeutic agent in breast cancer.

Keywords

Breast cancer miR-124 Ets-1 Proliferation Apoptosis Invasion 

Notes

Acknowledgments

This study was supported in part by Henan key programs for science and technology development (112101310500, 132102310093) and medical science and technology research programs in Henan Province (201201016).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Wentao Li
    • 1
  • Wenqiao Zang
    • 2
  • Pei Liu
    • 3
  • Yuanyuan Wang
    • 2
  • Yuwen Du
    • 2
  • Xiaonan Chen
    • 2
  • Meng Deng
    • 1
  • Wencong Sun
    • 1
  • Lei Wang
    • 1
  • Guoqiang Zhao
    • 2
  • Baoping Zhai
    • 1
  1. 1.Department of Breast SurgeryThe People’s Hospital of Henan Province (The People’s Hospital of Zhengzhou University)ZhengzhouChina
  2. 2.College of Basic Medical SciencesZhengzhou UniversityZhengzhouChina
  3. 3.School of Clinical MedicineZhengzhou UniversityZhengzhouChina

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