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Tumor Biology

, Volume 35, Issue 10, pp 9717–9723 | Cite as

Genome-wide screen for serum microRNA expression profile in mfat-1 transgenic mice

Research Article

Abstract

n-3 Polyunsaturated fatty acids (n-3 PUFAs) contribute to preventing many types of diseases, including cancer; however, a high n-6 polyunsaturated fatty acids (n-6 PUFAs) intake in modern diets has the opposite effect. Previously, we developed a transgenic mouse model that expresses a gene, fat-1, encoding an n-3 fatty acid desaturase, which converts n-6 PUFAs to n-3 PUFAs in vivo. MicroRNAs (miRNAs) in serum are stable, reproducible, and consistent among individuals of the same species and serve as potential biomarkers for the detection of cancers and other diseases. Employing illumina sequencing, we analyzed all the serum miRNAs in wild-type and mfat-1 transgenic mice. Using quantitative real-time PCR (RT-qPCR), we identified 12 miRNAs that were highly expressed in mfat-1 mice. Pathway analysis of targets regulated by these miRNAs revealed a significant number of genes involved in the development of cancer, including phosphatidylinositol 3-kinase (PI3K), mitogen-activated protein kinases (MAPK), and mammalian target of rapamycin (mTOR), which suggested a relationship between n-3 PUFAs and cancer prevention.

Keywords

mfat-1 transgenic mice Illumina sequencing miRNA n-3 PUFA Cancer 

Notes

Acknowledgments

This work was supported by a grant from Jiangsu Key Laboratory of Xenotransplantation (BM2012116).

Conflicts of interest

None

Supplementary material

13277_2014_2261_MOESM1_ESM.jpg (182 kb)
Supplement 1 (A) Partial gas chromatograph traces showing the polyunsaturated fatty-acid profiles of total lipids extracted from different groups of mice. The n-6 PUFA peak is marked in black while the n-3 PUFA peak is in red. The mice in all groups were fed a normal diet. (B) Measurement of n-6/ n-3 PUFA ratio in mouse tails (12-weeks-old) by gas chromatographic analysis. The compositions of n-6 or n-3 PUFAs are expressed using relative percentages; i.e., the distribution areas of n-3 or n-6 PUFA peaks divided by the total peak areas of all detectable saturated and unsaturated free fatty acids (from the same sample) resolved from the gas chromatography column. Values are the means ± SD (n=16) in different groups. (JPEG 181 kb)
13277_2014_2261_MOESM2_ESM.xlsx (39 kb)
Supplement 2 Illumina sequencing data of serum microRNAs (XLSX 39 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  1. 1.State Key Laboratory of Reproductive Medicine, Jiangsu Key Laboratory of XenotransplantationNanjing Medical UniversityNanjingChina

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