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Tumor Biology

, Volume 35, Issue 10, pp 9847–9853 | Cite as

RETRACTED ARTICLE: MicroRNA-32 inhibits osteosarcoma cell proliferation and invasion by targeting Sox9

  • Jian-Qiang Xu
  • Wei-Bin Zhang
  • Rong Wan
  • Yao-Qi Yang
Research Article

Abstract

Increasing reports suggest that discovery of microRNAs (miRNAs) might provide a novel therapeutical target for human cancers, including osteosarcoma. Previous studies have shown that miR-32 was dysregulated in breast and endometrial cancer. However, its biological roles in osteosarcoma remain unclear. In the current study, we found that miR-32 was significantly down-regulated in osteosarcoma tissues, compared with the adjacent normal tissues. In vitro studies further demonstrated that miR-32 mimics were able to suppress, while its antisense oligos promoted cell proliferation in Saos-2 and U2OS cells. At the molecular level, our data further revealed that expression of Sox9 was negatively regulated by miR-32. Therefore, our results identify an important role for miR-32 in the osteosarcoma through regulating Sox9 expression.

Keywords

Osteosarcoma Cell proliferation MicroRNA miR-32 Sox9 

Notes

Conflict of interest

None

Supplementary material

13277_2014_2229_MOESM3_ESM.jpg (82 kb)
Supplementary Fig. 1

Expression levels of PTEN in Saos-2 and U2OS cells. (A-B) Protein levels of PTEN were determined by western blot in Saos-2 (A) and U2OS (B) cells transfected with miR-32 mimics or negative controls (NC). (JPEG 81 kb)

13277_2014_2229_MOESM1_ESM.tif (140 kb)
High resolution image (TIFF 140 kb)
13277_2014_2229_MOESM4_ESM.jpg (107 kb)
Supplementary Fig. 2

Sox9 re-introduction blocks the anti-proliferative roles of miR-32 in U2OS cells. (A) Sox9 protein expression was determined by western blot in U2OS cells. Cells were pre-transfected with miR-32 mimics or negative control (NC) for 24 hr, and then transfected with lentiviruses containing Sox9 gene or empty vector (EV) for another 24 hr. (B-D) The growth curve (B), cell proliferation (BrdU, C) and invasion abilities (D) was determined in U2OS cells. *P < 0.05 **P < 0.01, ***P < 0.001 between two groups. (JPEG 107 kb)

13277_2014_2229_MOESM2_ESM.tif (435 kb)
High resolution image (TIFF 435 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Jian-Qiang Xu
    • 1
  • Wei-Bin Zhang
    • 1
  • Rong Wan
    • 1
  • Yao-Qi Yang
    • 1
  1. 1.Department of Orthopaedics, Ruijin Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina

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