Establishment of experimental implantation tumor models of hepatocellular carcinoma in Wistar rats
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Our aims were to investigate and establish simple and reliable implanted hepatocellular carcinoma (HCC) models in Wistar rats. Concentrated suspensions of CBRH-7919 cancer cell lines were injected subcutaneously into the scapular regions of nude mice. The developing tumor tissues were then implanted into the livers of 45 adult Wistar rats. Dexamethasone (2.5 mg/day) was injected intramuscularly daily for 1 week preoperatively and 2 weeks postoperatively. After 4 weeks of implantation, ultrasonography and nuclear magnetic resonance imaging (MRI) were performed to identify model rats with liver tumor growth and to analyze the growth and characteristics of the tumors. Five of these model rats were then sacrificed, and the tumors were removed from the liver for pathological examination. Three rats died during the operation; among the remaining 42 rats, 36 possessed a total of 43 liver tumors. The success rate of tumor implantation was 85.7 % (36/42), and the diameters of the tumors ranged from 5 to 10 mm. All tumor specimens were confirmed to be HCC by pathological examination. This study provides a new approach for establishing implanted HCC models in Wistar rats, which can be used for studying numerous biological features of HCC.
KeywordsHepatocellular carcinoma Wistar rat CBRH-7919 cell lines Animal models
This work was supported by the National Natural Science Foundation of China (Nos. 81171441, 81301694, 81371653), the Science and Technology Program of Guangdong Province (No. 2012B060300009), the Natural Science Foundation of Guangdong Province (Nos. S2012020010904, 7001663, S2011010004247), and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (No. 20111139).
Conflicts of interest
- 1.Crissien AM, Frenette C. Current management of hepatocellular carcinoma. Gastroenterol Hepatol (N Y). 2014;10:153–61.Google Scholar
- 8.Nishikawa H, Arimoto A, Wakasa T, Kita R, Kimura T, Osaki Y. Comparison of clinical characteristics and survival after surgery in patients with non-B and non-C hepatocellular carcinoma and hepatitis virus-related hepatocellular carcinoma. J Cancer. 2013;4:502–13.PubMedCentralCrossRefPubMedGoogle Scholar
- 20.Zhang HJ, Chen D, Tian JM. Establishment of implanted hepatic cancer model in rats under the guidance of B-ultrasound. Chin J Med Comput Imaging. 2005;11:122–4.Google Scholar
- 21.Wang SJ, Chen B. Establishment of transplanted hepatic cancer model in SD rats. Oncol Prog. 2009;7:571–3.Google Scholar
- 22.Chu TH, Yeh HJ, Chen JMI. Establishment and some biological characteristics of a rat hepatoma cell line (CBRH-7919). Acta Biol Exp Sin. 1980;13:113–5.Google Scholar