Tumor Biology

, Volume 35, Issue 8, pp 8143–8148 | Cite as

Chrebp regulates the transcriptional activity of androgen receptor in prostate cancer

  • Xue-Lei Wang
  • Xiao-Fei Wen
  • Rong-Bing Li
  • Bin Liu
  • Guang-Ming Qiu
  • Ji-Ling Wen
  • Yue-Min Wang
Research Article

Abstract

Androgen receptor (AR), a member of nuclear hormone receptor, plays an essential role in the initiation and progression of prostate cancer (PCa). In the present study, by way of immunoprecipitation followed by mass spectrometry (IP/MS) system, we found that carbohydrate-responsive element-binding protein (Chrebp), a glucose sensor in normal and cancer cells, interacted with AR in LNCaP cells. The interaction was further confirmed by coimmunoprecipitation analysis. Besides, Chrebp is required for the optimal transcriptional activity of AR in promoting the transcription of the prostate-specific antigen (PSA) promoter and messenger RNA (mRNA) expression. Consistently, knockdown of Chrebp using small interfering RNA (siRNA) in LNCaP cells reduced endogenous PSA levels. Together, our study demonstrates that Chrebp interacts with AR and regulates its transcriptional activity.

Keywords

Androgen receptor Prostate-specific antigen Carbohydrate-responsive element-binding protein Prostate cancer 

Notes

Acknowledgments

This work was supported by Dongfang Zhaoyang Talent Plan (DFZY-3).

Conflicts of interest

None.

Supplementary material

13277_2014_2085_Fig5_ESM.gif (247 kb)
Supplementary Fig. 1

(A-B) mRNA levels of FASN AND L-PK in LNCaP cells with Chrebp overexpression (A) or knockdown (B) (GIF 247 kb)

13277_2014_2085_MOESM1_ESM.tif (238 kb)
High resolution image(TIFF 237 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Xue-Lei Wang
    • 1
  • Xiao-Fei Wen
    • 1
  • Rong-Bing Li
    • 1
  • Bin Liu
    • 2
  • Guang-Ming Qiu
    • 1
  • Ji-Ling Wen
    • 1
  • Yue-Min Wang
    • 1
  1. 1.Department of Urology, East HospitalTongji University School of MedicineShanghaiPeople’s Republic of China
  2. 2.Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghaiPeople’s Republic of China

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