Tumor Biology

, Volume 35, Issue 8, pp 8209–8216 | Cite as

Aberrant promoter methylation of HIN-1 gene may contribute to the pathogenesis of breast cancer: a meta-analysis

  • Di Dai
  • Xi-Hua Dong
  • Shi-Tong Cheng
  • Ge Zhu
  • Xiao-Lin Guo
Research Article


We conducted the present meta-analysis of relevant cohort studies to evaluate whether promoter methylation of the high in normal-1 (HIN-1) gene contributes to breast cancer. The MEDLINE (1966 ~ 2013), Cochrane Library (Issue 12, 2013), EMBASE (1980 ~ 2013), CINAHL (1982 ~ 2013), Web of Science (1945 ~ 2013), and Chinese Biomedical (CBM) (1982 ~ 2013) databases were searched without any language restrictions. Meta-analyses were conducted using Stata software (version 12.0; Stata Corporation, College Station, TX, USA). Crude odds ratios (ORs) with their 95 % confidence interval (CI) were calculated. Nine clinical cohort studies that enrolled a total of 693 breast cancer patients were included in the meta-analysis. The results of our meta-analysis demonstrated that HIN-1 methylation frequency in cancer tissue was significantly higher than that of normal and benign tissues (cancer tissue vs. normal tissue: OR = 52.60, 95 % CI = 33.77 ~ 81.92, P < 0.001; cancer tissue vs. benign tissue: OR = 2.38, 95 % CI = 1.53 ~ 3.70, P < 0.001; respectively). Ethnicity-stratified analysis indicated that HIN-1 promoter methylation was correlated with the pathogenesis of breast cancer among both Asians and Caucasians (all P < 0.05). Our findings provide empirical evidence that aberrant HIN-1 promoter methylation may contribute to the pathogenesis of breast cancer. Thus, aberrant HIN-1 promoter methylation could be an independent and important biomarker used in predicting the prognosis and progression of breast cancer.


HIN-1 Promoter methylation Breast cancer Meta-analysis 



The study was funded by Specification of Individual Medical Testing, Standardization Research and Application (The 2014 annual public industry research) (201402018). We would like to acknowledge the helpful comments on this paper received from our reviewers.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Di Dai
    • 1
  • Xi-Hua Dong
    • 1
  • Shi-Tong Cheng
    • 1
  • Ge Zhu
    • 1
  • Xiao-Lin Guo
    • 1
  1. 1.Department of Laboratory MedicineThe First Hospital of China Medical UniversityShenyangPeople’s Republic of China

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