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Tumor Biology

, Volume 35, Issue 8, pp 7645–7650 | Cite as

miR-145 inhibits osteosarcoma cells proliferation and invasion by targeting ROCK1

  • Enqi Li
  • Jinli Zhang
  • Tianxiang Yuan
  • Baotong Ma
Research Article

Abstract

MicroRNAs (miRNAs) contribute to the development and progression of various types of human cancers. The aim of this study was to study the role of miR-145 and to identify its functional target gene in osteosarcoma (OS) cells. We found that miR-145 was reduced in OS tissues and cell lines. Enforced expression of miR-145 inhibited cell proliferation, migration, and invasion abilities of MG-63 cells. Furthermore, we revealed that Rho-associated protein kinase 1 (ROCK1) was a target of miR-145 in OS. Finally, we found that silencing of ROCK1 performed similar effects with miR-145 in MG-63 cells, and ROCK1 was inversely correlated with miR-145 in OS tissues. Collectively, these data indicate that miR-145 may act as a tumor suppressor and contributes to the progression of OS through targeting ROCK1.

Keywords

Osteosarcoma miR-145 ROCK1 Proliferation Invasion 

Notes

Conflicts of interest

None.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Enqi Li
    • 1
  • Jinli Zhang
    • 1
  • Tianxiang Yuan
    • 1
  • Baotong Ma
    • 1
  1. 1.Department of Orthopaedic TraumaTianJin HospitalTianjinChina

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