Tumor Biology

, Volume 35, Issue 8, pp 7645–7650 | Cite as

miR-145 inhibits osteosarcoma cells proliferation and invasion by targeting ROCK1

  • Enqi Li
  • Jinli Zhang
  • Tianxiang Yuan
  • Baotong Ma
Research Article


MicroRNAs (miRNAs) contribute to the development and progression of various types of human cancers. The aim of this study was to study the role of miR-145 and to identify its functional target gene in osteosarcoma (OS) cells. We found that miR-145 was reduced in OS tissues and cell lines. Enforced expression of miR-145 inhibited cell proliferation, migration, and invasion abilities of MG-63 cells. Furthermore, we revealed that Rho-associated protein kinase 1 (ROCK1) was a target of miR-145 in OS. Finally, we found that silencing of ROCK1 performed similar effects with miR-145 in MG-63 cells, and ROCK1 was inversely correlated with miR-145 in OS tissues. Collectively, these data indicate that miR-145 may act as a tumor suppressor and contributes to the progression of OS through targeting ROCK1.


Osteosarcoma miR-145 ROCK1 Proliferation Invasion 


Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Enqi Li
    • 1
  • Jinli Zhang
    • 1
  • Tianxiang Yuan
    • 1
  • Baotong Ma
    • 1
  1. 1.Department of Orthopaedic TraumaTianJin HospitalTianjinChina

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