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Tumor Biology

, Volume 35, Issue 8, pp 7775–7780 | Cite as

Association between miR-146aG>C and miR-196a2C>T polymorphisms and the risk of hepatocellular carcinoma in a Chinese population

  • Bing Zhou
  • Liang-peng Dong
  • Xiao-yue Jing
  • Jin-song Li
  • Shu-juan Yang
  • Jun-ping Wang
  • Long-feng Zhao
Research Article

Abstract

MicroRNAs have been demonstrated to have a role in susceptibility and prognosis of various types of human cancer. We investigated the association between polymorphisms in miR-146aG>C, miR-196a2C>T, and miR-499A>G and hepatocellular carcinoma (HCC) risk and interaction with HCC and hepatitis B virus (HBV) infection. Two hundred sixty-six cases with HCC and 281 health controls were enrolled in the present study. Genotyping of the miR-146aG>C, miR-196a2C>T, and miR-499A>G genotypes was conducted by duplex polymerase chain reaction with the confronting two-pair primer (PCR–RFLP). Subjects with miR-146a GG and G allele had an increased risk of HCC compared with the homozygote CC genotype. Similarly, HCC patients carrying microRNA (miRNA)-196a2 computed tomography, TT, and T allele significantly decreased the risk of HCC relative to the CC genotype. Stratified analysis indicated that miR-196a2C>T polymorphism was associated with reduced risk of HBV-related HCC, but not in hepatitis C virus- and nonviral-related HCC cases. In conclusion, miR-146aG>C and miR-196a2C>T polymorphism are associated with risk of HCC patients in China, especially in patients with HBV infection. SNPs in miRNA sequences can be used as a diagnostic biomarker for HCC.

Keywords

MicroRNA Polymorphisms Hepatocellular carcinoma HBV 

Notes

Acknowledgments

We thank the First Affiliated Hospital of Xinxiang Medical University.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Bing Zhou
    • 1
  • Liang-peng Dong
    • 1
  • Xiao-yue Jing
    • 1
  • Jin-song Li
    • 2
  • Shu-juan Yang
    • 3
  • Jun-ping Wang
    • 4
  • Long-feng Zhao
    • 5
  1. 1.Department of General SurgeryThe First Affiliated Hospital of Xinxiang Medical UniversityWeihuiChina
  2. 2.Department of Pathology DepartmentThe First Affiliated Hospital of Xinxiang Medical UniversityWeihuiChina
  3. 3.Department of Child, Adolescent and Maternal Health, West China School of Public HealthSichuan UniversityChengduChina
  4. 4.Shanxi Provincial People’s HospitalTaiyuanChina
  5. 5.The First of Hospital of Shanxi Medical UniversityTaiyuanChina

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