Tumor Biology

, Volume 35, Issue 11, pp 11193–11198 | Cite as

A4383C and C76G SNP in Cathepsin B is respectively associated with the high risk and tumor size of hepatocarcinoma

  • Tsung-Po Chen
  • Shun-Fa Yang
  • Chiao-Wen Lin
  • Hsiang-Lin Lee
  • Chiung-Man Tsai
  • Chia-Jui Weng
Research Article


Single nucleotide polymorphism (SNP) in some genes is a candidate for having or developing a cancer. Cathepsin B (CTSB) is considered to be the biomarker of cancers. The study aimed to evaluate the impacts of three SNPs in CTSB gene on the risk and progress of hepatocellular carcinoma (HCC). The SNPs of CTSB C76G (rs12338), CTSB A4383C (rs13332), and CTSB A8422G (rs8898) from 135 patients with HCC and 520 control participants in Taiwan were determined by real-time PCR. Through analyzing by statistics, we found that the polymorphism of rs13332 was significantly associated to the risk of HCC cancer; a significantly high frequent tumor size development was observed in HCC patients carrying rs12338 polymorphic genotype than those carrying ancestral genotype. The SNPs of rs12338, rs13332, and rs8898 were irrelevant to the frequencies of HCC clinical status and the levels of HCC clinicopathological markers. In conclusions, CTSB A4383C SNP is observed modestly more often in patients who developed HCC than in healthy controls and might be associated with the risk of HCC. The association between CTSB C76G SNP and greater tumor size may warrant further study in regards to the biology of HCC.


Cathepsin B Diagnostic marker Hepatocellular carcinoma Single nucleotide polymorphism 



Adjusted odds ratio


Confidence interval


Cathepsin B


Hepatocellular carcinoma


Polymerase chain reaction


Single nucleotide polymorphism



This study was supported by a research grant from Central Region Hospital Alliance, Ministry of Health and Welfare, Taiwan (2013 project number: 10215).

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Tsung-Po Chen
    • 1
    • 2
  • Shun-Fa Yang
    • 1
    • 2
  • Chiao-Wen Lin
    • 3
    • 4
  • Hsiang-Lin Lee
    • 1
    • 5
  • Chiung-Man Tsai
    • 1
    • 6
  • Chia-Jui Weng
    • 7
  1. 1.Institute of MedicineChung Shan Medical UniversityTaichungTaiwan
  2. 2.Department of Medical ResearchChung Shan Medical University HospitalTaichungTaiwan
  3. 3.Institute of Oral SciencesChung Shan Medical UniversityTaichungTaiwan
  4. 4.Department of DentistryChung Shan Medical University HospitalTaichungTaiwan
  5. 5.Department of SurgeryChung Shan Medical University HospitalTaichungTaiwan
  6. 6.Ministry of Health and WelfareTainan HospitalTainan CityTaiwan
  7. 7.Department of Living Services IndustryTainan University of TechnologyTainan CityTaiwan

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