Tumor Biology

, Volume 35, Issue 8, pp 7635–7644 | Cite as

Ki-67, TGF-β1, and elastin content are significantly altered in lip carcinogenesis

  • Gabriela Salvadori
  • Jean Nunes dos Santos
  • Marco Antonio Trevizani Martins
  • Artur Cunha Vasconcelos
  • Luise Meurer
  • Pantelis Varvaki Rados
  • Vinicius Coelho Carrard
  • Manoela Domingues Martins
Research Article


Epithelial changes observed in actinic cheilitis (AC) and lower lip squamous cell carcinoma (LLSCC) have been studied using different markers in order to observe diagnostic and prognostic factors for both lesions. The aim of the present study was to analyze Ki-67, TGF-β1, and elastin content in AC and LLSCC to determine the possible role of these proteins in lip carcinogenesis. Medical records of 29 cases of AC and 53 cases of LLSCC were analyzed. Lesions were classified according histological pattern and submitted to immunostaining for Ki-67, TGF-β1, and elastin. Different percentages of Ki-67-positive cells were found in AC depending on the degree of epithelial dysplasia (p < 0.01). An association was also found between the percentage of Ki-67-positive cells and tumor grade in LLSCC (p < 0.01). An inverse correlation was found between Ki-67 and TGF-β1 in AC and LLSCC (p < 0.01). Elastosis was thinner and more discontinuous in LLSCC in comparison to AC, and this difference in the elastin immunolabeling pattern was statistically significant between groups (p < 0.01). The present findings indicate that changes in Ki-67 and TGF-β1 content contribute to lip carcinogenesis. Furthermore, elastin content reflects changes in the extracellular matrix in both AC and LLSCC.


Oral cancer Prognostic biomarkers Potentially malignant disorders 



This study was supported by the Postgraduate Research Group of the Porto Alegre University Hospital (GPPG/FIPE: 12–0176). Santos JN, Meurer L, and Martins MD are research fellows at Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil. The author’s would like to thank Dr. Pablo Agustin Vargas (FOP-UNICAMP) for his help with photomicrographs.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Gabriela Salvadori
    • 1
  • Jean Nunes dos Santos
    • 2
  • Marco Antonio Trevizani Martins
    • 1
  • Artur Cunha Vasconcelos
    • 1
  • Luise Meurer
    • 3
  • Pantelis Varvaki Rados
    • 1
  • Vinicius Coelho Carrard
    • 1
  • Manoela Domingues Martins
    • 1
    • 4
  1. 1.Department of Oral Pathology, School of DentistryFederal University of Rio Grande do SulPorto AlegreBrazil
  2. 2.Department of Oral Pathology, Laboratory of Oral Surgical Pathology, School of DentistryFederal University of BahiaSalvadorBrazil
  3. 3.Department of Pathology, School of MedicineFederal University of Rio Grande do SulPorto AlegreBrazil
  4. 4.School of Dentistry, Oral Pathology DepartmentFederal University of Rio Grande do SulSantana, Porto AlegreBrazil

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