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MicroRNA-195 regulates steroid receptor coactivator-3 protein expression in hepatocellular carcinoma cells

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Tumor Biology

Abstract

Multiple studies have shown that steroid receptor coactivator-3 (SRC-3) is upregulated and promotes cell proliferation in several human cancers, including breast, lung, and prostate carcinoma. However, its molecular determinants remain largely unexplored. In the current study, by way of informatics software, we found that MicroRNA-195 (miR-195) could negatively regulate protein levels of SRC-3 through targeting its 3′-untranslated region (3′-UTR) in hepatocellular carcinoma (HCC) cells. As a result, miR-195 mimics inhibited while its antisense enhanced SRC-3 protein levels. Furthermore, miR-195 could modulate cell proliferation and tumor growth in vivo and in vitro. Therefore, our results demonstrate a novel molecular mechanism for the dysregulated expression of SRC-3 in hepatocellular carcinoma.

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Acknowledgments

This work was supported by the Science and Technology Program of LiaoNing (2012225019).

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Authors and Affiliations

  1. The Fourth Affiliated Hospital of China Medical University, Shenyang, China

    Hong-Lei Jiang, Hao Yu, Xu Ma, Dong Xu, Guo-Fu Lin, Dong-Yan Ma & Jun-Zhe Jin

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  1. Hong-Lei Jiang
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  2. Hao Yu
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  3. Xu Ma
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  4. Dong Xu
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  5. Guo-Fu Lin
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  6. Dong-Yan Ma
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Corresponding author

Correspondence to Jun-Zhe Jin.

Electronic supplementary material

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Supplementary Fig. 1

(a-b) Western blot analysis of SRC-3 expression in HepG2 cells with or without transfection of miR-195 mimics (a), antisense (b) or negative control (NC). (TIFF 216 kb)

Supplementary Fig. 2

(a-b) Western blot analysis of SRC-3 expression in HuH7 cells transfected with miR-195 mimics (a), antisense (b) or negative control (NC). (TIFF 137 kb)

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Jiang, HL., Yu, H., Ma, X. et al. MicroRNA-195 regulates steroid receptor coactivator-3 protein expression in hepatocellular carcinoma cells. Tumor Biol. 35, 6955–6960 (2014). https://doi.org/10.1007/s13277-014-1933-x

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  • DOI: https://doi.org/10.1007/s13277-014-1933-x

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