Tumor Biology

, Volume 35, Issue 7, pp 6955–6960 | Cite as

MicroRNA-195 regulates steroid receptor coactivator-3 protein expression in hepatocellular carcinoma cells

  • Hong-Lei Jiang
  • Hao Yu
  • Xu Ma
  • Dong Xu
  • Guo-Fu Lin
  • Dong-Yan Ma
  • Jun-Zhe Jin
Research Article

Abstract

Multiple studies have shown that steroid receptor coactivator-3 (SRC-3) is upregulated and promotes cell proliferation in several human cancers, including breast, lung, and prostate carcinoma. However, its molecular determinants remain largely unexplored. In the current study, by way of informatics software, we found that MicroRNA-195 (miR-195) could negatively regulate protein levels of SRC-3 through targeting its 3′-untranslated region (3′-UTR) in hepatocellular carcinoma (HCC) cells. As a result, miR-195 mimics inhibited while its antisense enhanced SRC-3 protein levels. Furthermore, miR-195 could modulate cell proliferation and tumor growth in vivo and in vitro. Therefore, our results demonstrate a novel molecular mechanism for the dysregulated expression of SRC-3 in hepatocellular carcinoma.

Keywords

SRC-3 miR-195 Hepatocellular carcinoma Proliferation 

Notes

Acknowledgments

This work was supported by the Science and Technology Program of LiaoNing (2012225019).

Conflicts of interest

None

Supplementary material

13277_2014_1933_MOESM1_ESM.tif (217 kb)
Supplementary Fig. 1 (a-b) Western blot analysis of SRC-3 expression in HepG2 cells with or without transfection of miR-195 mimics (a), antisense (b) or negative control (NC). (TIFF 216 kb)
13277_2014_1933_MOESM2_ESM.tif (137 kb)
Supplementary Fig. 2 (a-b) Western blot analysis of SRC-3 expression in HuH7 cells transfected with miR-195 mimics (a), antisense (b) or negative control (NC). (TIFF 137 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Hong-Lei Jiang
    • 1
  • Hao Yu
    • 1
  • Xu Ma
    • 1
  • Dong Xu
    • 1
  • Guo-Fu Lin
    • 1
  • Dong-Yan Ma
    • 1
  • Jun-Zhe Jin
    • 1
  1. 1.The Fourth Affiliated Hospital of China Medical UniversityShenyangChina

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