Tumor Biology

, Volume 35, Issue 7, pp 6763–6768 | Cite as

Diagnostic value of CD117 in differential diagnosis of acute leukemias

  • Abbas Ahmadi
  • Ali-Akbar Poorfathollah
  • Mahnaz Aghaiipour
  • Mansour Rezaei
  • Mahin Nikoo-ghoftar
  • Mohammad Abdi
  • Alireza Gharib
  • Amir Amini
Research Article


C-kit receptor (CD117) and its ligand, stem cell factor, play a key role in normal hematopoiesis. It has been demonstrated that its expression extremely increases in leukemias with myeloid commitment. We analyzed findings on CD117 expression together with other myeloid related markers in 203 de novo acute leukemias, referred to Iranian immunophenotyping centers: Iranian Blood Transfusion Organization (IBTO) and Baghiatallah Hospital (BH). All cases were characterized based on the French American British cooperative group (FAB) and European Group for Immunological Classification of Leukemias (EGIL). The cases comprised of 111 acute myeloblastic leukemia (AML), 86 acute lymphoblastic leukemia (ALL), and 6 acute undifferentiated leukemia (AUL). CD117 was positive in 75 % of AML and 50 % of AUL, whereas none of the ALL cases was positive for this marker. Although CD117 was positive in 100 % of M5a cases, no M5b positive was found (p = 0.036). The calculated specificity for myeloid involvement was 100 % for CD117 and CD33, and 98 % for CD13 and CD15 (p < 0.001). The calculated sensitivity for myeloid involvement was 83, 76, 64, and 41 % for CD13, CD117, CD33, and CD15, respectively (p < 0.001). We concluded that CD117 expression is a specific and rather sensitive marker for differential diagnosis between AML and ALL, and except for M5 subtypes, it fails to determine FAB subtypes; lack of expression in M5 can identify M5b. Therefore, it should be included in the routine primary panel for diagnosis of acute leukemias.


Acute Leukemia Immunophenotyping CD117 AML ALL 



The authors wish to thank all the patients for participating in the study. Financial supports for this study were provided by the Iranian Blood Transfusion Organization, Tehran, Iran.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Abbas Ahmadi
    • 1
  • Ali-Akbar Poorfathollah
    • 2
  • Mahnaz Aghaiipour
    • 3
  • Mansour Rezaei
    • 4
  • Mahin Nikoo-ghoftar
    • 3
  • Mohammad Abdi
    • 5
  • Alireza Gharib
    • 6
  • Amir Amini
    • 7
  1. 1.Cellular and Molecular Research CenterKurdistan University of Medical SciencesSanandajIran
  2. 2.Immunology DepartmentTarbiat Modares UniversityTehranIran
  3. 3.Flowcytometry DepartmentIranian Blood Transfusion OrganizationTehranIran
  4. 4.Biostatistics DepartmentKermanshah University of Medical SciencesKermanshahIran
  5. 5.Department of Pathology and Medical Laboratory Sciences, Para-Medicine FacultyKurdistan University of Medical SciencesSanandajIran
  6. 6.Deputy of Research and TechnologyKurdistan University of Medical SciencesSanandajIran
  7. 7.Exercise Physiology Research CenterBaqiyatallah University of Medical SciencesTehranIran

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