Tumor Biology

, Volume 35, Issue 7, pp 6485–6492 | Cite as

Capsaicin inhibits cell proliferation by cytochrome c release in gastric cancer cells

  • Ogunc Meral
  • Merve Alpay
  • Gorkem Kismali
  • Funda Kosova
  • Dilek Ulker Cakir
  • Mert Pekcan
  • Serbulent Yigit
  • Tevhide Sel
Research Article

Abstract

Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide) is the principal pungent component in hot peppers. The role of capsaicin in carcinogenesis is quite controversial. Although some investigators suspect that capsaicin is a carcinogen, co-carcinogen, or tumor promoter, others have reported that it has chemopreventive and chemotherapeutic effects. The present study aimed to evaluate the cytotoxicity and chemosensitizing activities of capsaicin alone and on 5-flourouracil (5-FU)-treated gastric cancer cells. In this study, the gastric cancer cell line HGC-27 was used and capsaicin used as a chemosensitizer and 5-flourouracil (5-FU) was used as chemotherapeutic. Cytotoxicity and chemosensitizing activities were analyzed with MTT assay; supernatant levels of LDH and glucose were detected as biochemical markers of cell viability; cytochrome c and AIF were evaluated with western blot; and additionally, wound-healing assays were employed. Results suggested that capsaicin had significant anticancer abilities; such capsaicin were capable of causing multifold decreases in the half maximal inhibitory concentration IC50 value of 5-FU. The continuing controversy surrounding consumption or topical application of capsaicin clearly suggests that more well-controlled epidemiologic studies are needed to evaluate the safety and efficacy of capsaicin use. In summary, the present study demonstrated that capsaicin has the potential to be used for treating gastric carcinoma with 5-FU in vitro.

Keywords

Capsaicin 5-Fluorouracil Gastric cancer Cytochrome c 

Notes

Acknowledgments

Authors sincerely thank Dr. Begum Yurdakok from the Department of Pharmacology, Ankara University.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Ogunc Meral
    • 1
  • Merve Alpay
    • 1
    • 4
  • Gorkem Kismali
    • 1
  • Funda Kosova
    • 2
  • Dilek Ulker Cakir
    • 3
  • Mert Pekcan
    • 1
  • Serbulent Yigit
    • 5
  • Tevhide Sel
    • 1
  1. 1.Faculty of Veterinary Medicine, Department of BiochemistryAnkara UniversityAnkaraTurkey
  2. 2.Celal Bayar UniversityManisaTurkey
  3. 3.Faculty of Medicine, Department of BiochemistryCanakkale Onsekiz Mart UniversityCanakkaleTurkey
  4. 4.Faculty of MedicineDuzce UniversityDuzceTurkey
  5. 5.Faculty of Medicine, Department of Medical BiologyGaziosmanpasa UniversityTokatTurkey

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