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Tumor Biology

, Volume 35, Issue 7, pp 6467–6474 | Cite as

Rapamycin combined with celecoxib enhanced antitumor effects of mono treatment on chronic myelogenous leukemia cells through downregulating mTOR pathway

  • Jie Li
  • Liying Xue
  • Hongling Hao
  • Ruoyu Li
  • Jianmin Luo
Research Article

Abstract

Chronic myelogenous leukemia is a neoplasm of myeloid progenitor cells. We recently found that rapamycin could induce G0/G1 phase arrest and apoptosis and inhibit proliferation of K562 cells through inhibiting mammalian target of rapamycin (mTOR) pathway. However, whether rapamycin has synergistic effects with other drugs in chronic myelogenous leukemia (CML) therapies remain unclear. Therefore, we examined the effect of rapamycin combined with celecoxib on K562 cells in vitro. The survival rates showed a significant decrease in rapamycin + celecoxib treatment group. The combination treatment also increased the G0/G1 phase cells as compared to rapamycin or celecoxib treatment alone (P < 0.05), accompanied with the decreased population of S phase cells. Meanwhile, the rate of apoptosis was 15.87 ± 2.21 % in rapamycin + celecoxib treatment group, significantly higher than that in mono treatment group (P < 0.05). Western blot and reverse transcription PCR (RT-PCR) analysis showed that the expressions of mTOR, 4E-BP1, and p70S6K were all significantly decreased in K562 cells after rapamycin + celecoxib treatment (P < 0.05). In conclusion, rapamycin combined with celecoxib could induce cell cycle arrest and apoptosis and decrease the expressions of mTOR, 4E-BP1, and p70S6K. It suggested that the combination could enhance the antitumor effects of mono treatment on CML cells through downregulating mTOR pathway.

Keywords

Chronic myelogenous leukemia mTOR Rapamycin Celecoxib 

Notes

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Jie Li
    • 1
  • Liying Xue
    • 2
  • Hongling Hao
    • 1
  • Ruoyu Li
    • 3
  • Jianmin Luo
    • 4
  1. 1.Department of HematologyHebei General HospitalShijiazhuangChina
  2. 2.Department of PathologyHebei Medical UniversityShijiazhuangChina
  3. 3.Graduate FacultyHebei Medical UniversityShijiazhuangChina
  4. 4.Department of Hematology, The Second HospitalHebei Medical UniversityShijiazhuangChina

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