Tumor Biology

, Volume 35, Issue 6, pp 6113–6118 | Cite as

p16 CDKN2A SNP rs11515 was not associated with head and neck carcinoma

  • Ugo Borges Pinheiro
  • Carlos Alberto de Carvalho Fraga
  • Danilo Cangussu Mendes
  • Luciano Marques-Silva
  • Lucyana Conceição Farias
  • Marcela Gonçalves de Souza
  • Mariana Batista Soares
  • Kimberly Marie Jones
  • Sérgio Henrique Souza Santos
  • Alfredo Maurício Batista de Paula
  • Gustavo Velásquez-Meléndez
  • André Luiz Guimarães
Research Article


Head and neck squamous cell carcinoma (HNSCC) is considered a serious public health problem in many countries. Recently, genetic variations have been considered as important factors to cancer susceptibility and prognosis. More specifically, genetic polymorphisms have been associated with the development and prognosis of HNSCC. The purpose of the current study was to investigate an association among p16 CDKN2A gene polymorphism at rs11515, age, and HNSCC aggressiveness. PCR–RFLP analysis was used to investigate the p16 CDKN2A gene in 96 patients with HNSCC and in 100 individuals without HNSCC. A case group was categorized by age in younger (<60 years) and older (≥60 years) patients. Differences between the case and control groups were determined using Fisher and chi-squared tests. Time of survival was calculated from the date of diagnosis to the date of last follow-up visit or to the date of death using the Kaplan–Meier estimator and comparing this to the log-rank test. Statistical significance was set at p < 0.05. In the present study, no association was established between HNSCC and rs11515 polymorphism, as indicated in a previous study. We found that HNSCC individuals with large-sized tumors and with metastatic disease presented worse overall survival, consistent with fundamental concepts that establish the effects of tumor size and lymph node metastasis to HNSCC outcomes. This study identified that there is no difference in the distribution of rs11515 between the control and HNSCC groups. In addition, no differences between rs11515 genotypes and clinicopathological parameters were observed.


Suppressor gene CDKN2A Polymorphism Cancer Genetic 



This study was supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG). Dr. Guimarães, Dr. Santos, and Dr. de Paula are research fellows of CNPq.

Conflicts of interests


Ethical statement

All authors abide by the Association for Medical Ethics’ ethical rules of disclosure.


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Ugo Borges Pinheiro
    • 1
  • Carlos Alberto de Carvalho Fraga
    • 1
  • Danilo Cangussu Mendes
    • 1
  • Luciano Marques-Silva
    • 1
  • Lucyana Conceição Farias
    • 1
  • Marcela Gonçalves de Souza
    • 1
  • Mariana Batista Soares
    • 1
  • Kimberly Marie Jones
    • 2
  • Sérgio Henrique Souza Santos
    • 3
  • Alfredo Maurício Batista de Paula
    • 1
  • Gustavo Velásquez-Meléndez
    • 4
  • André Luiz Guimarães
    • 1
  1. 1.Department of DentistryUniversidade Estadual de Montes Claros, Hospital Universitário Clemente de FariaMontes ClarosBrazil
  2. 2.Program in Health SciencesUniversidade Estadual de Montes ClarosMontes ClarosBrazil
  3. 3.Department PharmacologyUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  4. 4.Department of NursingUniversidade Federal de Minas GeraisBelo HorizonteBrazil

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