Tumor Biology

, Volume 35, Issue 6, pp 5607–5612 | Cite as

Cyclin D1 G870A polymorphism and the risk of hepatocellular carcinoma in a Chinese population

  • Zhangyong Hu
  • Zhipeng Zhou
  • Guolian Xiong
  • Yali Wang
  • Yi Lai
  • Lan Deng
  • Jinliang Yang
Research Article

Abstract

Cyclin D1, encoded by the gene CCND1, is a regulatory protein in the cell cycle transition from G1 phase to S phase. A common polymorphism (G870A) in the exon 4 of CCND1 gene affects splicing of the CCND1 transcript and may cause uncontrollable cellular growth. Therefore, the CCND1 G870A polymorphism may influence an individual’s susceptibility to the development of certain tumors. The present study was performed to test the association between G870A polymorphism in the CCND1 gene and hepatocellular carcinoma (HCC) risk in a Chinese population. We extracted the peripheral blood samples from 220 patients with HCC and 220 age- and gender-matched healthy controls. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and direct DNA sequencing were performed to detect the polymorphism. The CCND1 genotype distribution among HCC patients was not significantly different from that among healthy controls (P = 0.08). Compared with the wild-type GG genotype, neither the variant AA genotype nor the variant genotypes containing the A allele were associated with risk of HCC. However, stratification analysis by HBV carrier status revealed that the variant genotypes containing the A allele were associated with a significantly increased risk of HCC among HBsAg-positive individuals (adjusted OR = 3.87; 95 % CI = 1.12, 13.30). These results suggest that the CCND1 G870A polymorphism may increase the risk of HBV-related HCC in the Chinese population.

Keywords

Hepatocellular carcinoma Cyclin D1 Polymorphism Case–control study 

Abbreviations

CCND1

Cyclin D1

OR

Odds ratio

CI

Confidence interval

PCR-RFLP

Polymerase chain reaction-restriction fragment length polymorphism

SNP

Single nucleotide polymorphisms

HWE

Hardy–Weinberg equilibrium

Notes

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Zhangyong Hu
    • 1
  • Zhipeng Zhou
    • 2
  • Guolian Xiong
    • 3
  • Yali Wang
    • 3
  • Yi Lai
    • 4
  • Lan Deng
    • 3
  • Jinliang Yang
    • 1
  1. 1.State Key Laboratory of Biotherapy and Cancer Center, West China HospitalWest China Medical School, Sichuan UniversityChengduPeople’s Republic of China
  2. 2.Department of Liver and Vascular SurgeryWest China Hospital, Sichuan UniversityChengduPeople’s Republic of China
  3. 3.Department of Infectious DiseaseThe First Affiliated Hospital of Chengdu Medical CollegeChengduPeople’s Republic of China
  4. 4.Department of Clinical Laboratory MedicineChengdu Medical CollegeChengduPeople’s Republic of China

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