Tumor Biology

, Volume 35, Issue 6, pp 5493–5500 | Cite as

RETRACTED ARTICLE: Canine transmissible venereal tumor and seminoma: a cytohistopathology and chemotherapy study of tumors in the growth phase and during regression after chemotherapy

  • J. Javanbakht
  • B. Pedram
  • M. R. Taheriyan
  • F. Khadivar
  • S. H. Hosseini
  • F. S. Abdi
  • E. Hosseini
  • M. Moloudizargari
  • S H. Aghajanshakeri
  • S. Javaherypour
  • R. Shafiee
  • R. Emrani Bidi
Research Article


In this study, 12 dogs affected by canine transmissible venereal tumor (CTVT) and testicular seminoma tumor were studied retrospectively. The cytological sample was smeared onto a glass slide and either air-dried for May-Grünwald-stain, and masses were surgically removed. The tumors were grossly examined, and sections of 4-μm thick were obtained from each sample and stained with H&E. For chemotherapy, vincristine sulfate was administered weekly as an infusion over 3 min via the cephalic vein at a dose of 0.025 mg/kg after diluting with physiological saline to a total amount of 10 ml. If no remission was observed after 8 weeks, chemotherapy was continued with weekly doxorubicin infusion at a dose of 1 mg/kg. All the tumor samples were divided into four cytohistopathologic groups, namely: multilobular (six cases), papillary (two cases), pedunculated (two cases), and tubular (two cases of seminoma). The most frequently represented tumor type was multilobular (6/10, 60 %) followed by pedunculated (2/10, 20 %), papillary (2/10, 20 %), and tubular (two cases of seminoma, 100 %). Cytological smears from eight tumors in regression after chemotherapy were poorly cellular, and many cells were fragmented. In two progressive tumors, there was an average of 1,406 ± 972 CTVT 200 cells/μl or 96 · 71 % of total cells counted. Thus, tumor cells represented 96 · 71 % of total cells within the biopsy specimens and the leukocytes 4 · 29 % (leukocyte, tumor cell ratio = 0 · 062 ± 0 · 031). In eight regressive tumors, there was an average of 1,245 ± 1,032 CTVT 200 cells/μl or 97 · 31 % of total cells counted. Thus, tumor cells represented 97 · 31 % of total cells and leukocytes 2 · 69 % (leukocyte, tumor cell ratio = 0 · 071 ± 0 · 174). Our data suggested that combination treatment with vincristine and doxorubicin in the future could be an excellent therapeutic alternative for the treatment of TVT for probably reducing the resistance to vincristine, and also, treatment success could easily be followed by the cytological changes.


Chemotherapy TVT Seminoma Cytology Pathology Canine 



The authors thank Dr. Saeid Fathi and Dr. Mehdi Aghamohammad Hassan, Faculty of Veterinary Medicine, Tehran University, Iran, for their help with this manuscript.

Conflicts of interest



  1. 1.
    Adami HO, Bergström R, Möhner M, Zatoński W, Storm H, Ekbom A, et al. Testicular cancer in nine northern European countries. Int J Cancer. 1994;59(1):33–8.CrossRefPubMedGoogle Scholar
  2. 2.
    Zheng T, Holford TR, Ma Z, Ward BA, Flannery J, Boyle P. Continuing increase in incidence of germ-cell testis cancer in young adults: experience from Connecticut, USA, 1935–1992. Int J Cancer. 1996;65(6):723–9.CrossRefPubMedGoogle Scholar
  3. 3.
    Schlottauer CF, Mcdonald JR, Bollman SL. Testicular tumours in dogs. J Urol. 1938;40:539–50.Google Scholar
  4. 4.
    Gurariĭ LL, Volkova MI, Khalaf’ian EA, Zakharova TI. Testicular sertolioma. Urologiia. 2002;4:10–4.Google Scholar
  5. 5.
    Ladds PW. The male genital system. In: Jubb KWF, Kennedy PC, Palmer N, editors. Pathology of Domestic Animals, vol. 3. 4th ed. San Diego: Academic; 1993. p. 504–5.Google Scholar
  6. 6.
    Peters MA, de Rooij DG, Teerds KJ, van Der Gaag I, van Sluijs FJ. Spermatogenesis and testicular tumours in ageing dogs. J Reprod Fertil. 2000;120(2):443–52.CrossRefPubMedGoogle Scholar
  7. 7.
    Bush JM, Gardiner DW, Palmer JS, Rajpert-De Meyts E, Veeramachaneni DN. Testicular germ cell tumours in dogs are predominantly of spermatocytic seminoma type and are frequently associated with somatic cell tumours. Int J Androl. 2011;34(42):e288–95.CrossRefPubMedGoogle Scholar
  8. 8.
    McGlynn KA, Devesa SS, Sigurdson AJ, Brown LM, Tsao L. Trends in the incidence of testicular germ cell tumors in the United States. Cancer. 2003;97:63–70.CrossRefPubMedGoogle Scholar
  9. 9.
    Maiolino P, Restucci B, Papparella S, Paciello O, De Vico G. Correlation of nuclear morphometric features with animal and human World Health Organization International Histological Classifications of canine spontaneous seminomas. Vet Pathol. 2004;41(6):608–11.CrossRefPubMedGoogle Scholar
  10. 10.
    MacLachlan, NJ, Kennedy PC. Tumours of the genital systems, In: tumors in domestic animals, 4th edn., Meuten, D.J., Ed., Iowa State Press 2002: 563–564.Google Scholar
  11. 11.
    Cheng L. Establishing a germ cell origin for metastatic tumors using OCT4 immunohistochemistry. Cancer. 2004;101(9):2006–10.CrossRefPubMedGoogle Scholar
  12. 12.
    Benazzi C, Sarli G, Preziosi R, Marcato PS. Laminin expression in testicular tumours of the dog. J Comp Pathol. 1995;112(2):141–50.CrossRefPubMedGoogle Scholar
  13. 13.
    Reif JS, Maguire TG, Kenney RM, Brodey RS. A cohort study of canine testicular neoplasia. J Am Vet Med Assoc. 1979;175(7):719–23.PubMedGoogle Scholar
  14. 14.
    Mostofi, FK, Sesterhenn, IA. Histological typing of testis tumors. In: World Health Organization International Histological Classification of Tumors, 2nd edn., World Health Organization, Geneva 1998: 10–12.Google Scholar
  15. 15.
    Nochomovitz LE, Rosai J. Current concepts on the histogenesis, pathology, and immunochemistry of germ cell tumors of the testis. Pathol Annu. 1978;1:327–62.Google Scholar
  16. 16.
    Feldman EC, Nelson RW. Canine and feline endocrinology and reproduction. Philadel-phia, PA: W. B. Saunders Company; 1987. p. 475–7.Google Scholar
  17. 17.
    Das U, Das AK. Review of canine transmissible venereal sarcoma. Vet Res Commun. 2000;24:545–6.CrossRefPubMedGoogle Scholar
  18. 18.
    Cohen D. The transmissible venereal tumor of the dog a naturally occurring allograft?: a review. Isr J Med Sci. 1978;14(1):14–9.PubMedGoogle Scholar
  19. 19.
    Boscos CM, Ververidis HN. Canine TVT—clinical findings, diagnosis and treatment. In Proceedings of the 29th World Small Animal Veterinary Association, Oct 6–9. Rhodes, Greece. [Online]. Available from [2011, 29, December].2004.
  20. 20.
    Mello Martins MI, Ferreira de Souza F, Gobello C. The canine transmissible venereal tumor: etiology, pathology, diagnosis and treatment. In: recent advances in small animal reproduction. P.W. Concannon, G. England, J. Veretgegen, C. Linde-Forsberg (eds). International Veterinary Information Service, Ithaca NY ( Apr 25, 2005: A1233.0405.
  21. 21.
    Amber EI, Henderson RA, Adeyanju JB, Gyang EO. Single-drug chemotherapy of canine transmissible venereal tumour with cyclophosphamide, methotrexate or vincristine. J Vet Intern Med. 1990;4:144–7.CrossRefPubMedGoogle Scholar
  22. 22.
    Singh J, Rana JS, Sood N, Pangawkar GR, Gupta PP. Clinico-pathological studies on the effect of different anti-neoplastic chemotherapy regimens on transmissible venereal tumours in dogs. Vet Res Commun. 1996;20:71–81.CrossRefPubMedGoogle Scholar
  23. 23.
    Rogers KS, Walker MA, Dillon HB. Transmissible venereal tumor: a retrospective study of 29 cases. J Am Anim Hosp Assoc. 1998;34:463–9.CrossRefPubMedGoogle Scholar
  24. 24.
    Kunakornsawat S, Yippaditr W, Jamjan N, Bootcah R, Netramai S, Viriyarumpa J, Kornkaewrat K. Surgical correction of transmissible venereal tumor with vincristine-resistance using episiotomy and vulvovaginoplasty in female and subtotal penile amputation and scrotal ablation in male dogs. In Proceeding of 48th Kasetsart University Annual Conference: Veterinary Medicine. Feb 3–5, Bangkok, Thailand.2010: 191–200.Google Scholar
  25. 25.
    Allen SW, Prasse KW, Mahaffey EA. Cytologic differentiation of benign from malignant canine mammary tumours. Vet Pathol. 1986;23:649–55.PubMedGoogle Scholar
  26. 26.
    Bancroft JD, Stevens A. Theory and practice of histological techniques. 4th ed. London: Churchill Livingstone; 1996. p. 276.Google Scholar
  27. 27.
    Shafiee R, Javanbakht J, Atyabi N, Kheradmand P, Kheradmand D, Bahrami A, Daraei H, Khadivar F. Diagnosis, classification and grading of canine mammary tumours as a model to study human breast cancer: a clinico-cytohistopathological study with environmental factors influencing public health and medicine. Cancer Cell Int. 2013; 9; 13(1):79.Google Scholar
  28. 28.
    Park MS, Kim Y, Kang MS, Oh SY, Cho DY, Shin NS, Kim DY. Disseminated transmissible venereal tumor in a dog. Journal of Veterinary Diagnostic Investigation 2006;18 (1)1: 130–133.CrossRefPubMedGoogle Scholar
  29. 29.
    Kroger D, Grey RM, Boyd JW. An unusual presentation of canine transmissible venereal tumor. Canine Practice Santa Barbara. 1991;16(6):17–21.Google Scholar
  30. 30.
    Brandão CVS, Borges AG, Ranzani JJT, Rahal SC, Teixeira CR, Rocha NS. Tumor venéreo transmissível: estudo retrospectivo de 127 casos (1998–2000). Rev Educ Contin CRMV-SP. 2002;5(1):25–31.Google Scholar
  31. 31.
    Amaral AS, Gaspar LFJ, Bassani-Silva S, Rocha NS. Diagnóstico citológico do tumor venéreo transmissível na região de Botucatu, Brasil (estudo descritivo: 1994–2003). Revista Portuguesa de Ciências Veterinárias. 2004;99(551):167–71.Google Scholar
  32. 32.
    Erünal-Maral N, Findik M, Aslan S. Use of exfoliative cytology for diagnosis of transmissible venereal tumour and controlling the recovery period in the bitch. Dtsch Tierarztl Wochenschr. 2000;107(5):175–80.PubMedGoogle Scholar
  33. 33.
    Bastan A, Baki Acar D, Cengiz M. Uterine and ovarian metastasis of transmissible venereal tumor in a bitch. Turk J Vet Anim Sci. 2008;32:65–6.Google Scholar
  34. 34.
    Stockmann D, Ferrari HF, Andrade AL, Lopes RA, Cardoso TC, Luvizotto Maria CR. Canine transmissible venereal tumors: aspects related to programmed cell death. Braz J Vet Pathol. 2011;4(1):67–75.Google Scholar
  35. 35.
    Galofaro V, Consiglio C, Rapisarda G, Marino F. Bilateral malignant seminoma with metastases in the mule: a report of two cases. Reprod Domest Anim. 2008;43:121–3.PubMedGoogle Scholar
  36. 36.
    Henson KL. Reproductive system. In: Raskin R, Meyer D, editors. Atlas of canine and feline cytology. Firstth ed. Philadelphia: W. B. Saunders; 2001.Google Scholar
  37. 37.
    Caraway NP, Fanning CV, Amato RJ, Sneige N. Fine needle aspiration cytology of seminoma: a review of 16 cases. Diagn Cytopathol. 1995;12:327–33.CrossRefPubMedGoogle Scholar
  38. 38.
    DeMay R. The art and science of cytopathology. Chicago, IL: ASCP Press; 1996. Aspiration Cytology; vol II 1996.Google Scholar
  39. 39.
    Cooley DM, Waters DJ. Small animal clinical oncology. In: Withrow SJ, MacEwen EG, editors. Tumors of the male reproductive system. 3rd ed. Philadelphia: Saunders; 2001. p. 478–89.Google Scholar
  40. 40.
    Das U, Das AK, Das D, Das BB. Clinical report on the efficacy of chemotherapy in canine transmissible venereal sarcoma. Indian Vet J. 2009;68:249–52.Google Scholar
  41. 41.
    Mozos E, Mendez A, Gomez-Villamandos JC. Immunohistochemical characterization of canine transmissible venereal tumor. Vet Pathol. 1996;33:257–63.CrossRefPubMedGoogle Scholar
  42. 42.
    Pérez J, Bautista MJ, Carrasco L, Gómez-Villamandos JC, Mozos E. Primary extragenital occurrence of transmissible venereal tumors: three case reports. Canine Pract. 1994;19(1):7–10.Google Scholar

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • J. Javanbakht
    • 1
  • B. Pedram
    • 2
  • M. R. Taheriyan
    • 3
  • F. Khadivar
    • 4
  • S. H. Hosseini
    • 5
  • F. S. Abdi
    • 6
  • E. Hosseini
    • 7
  • M. Moloudizargari
    • 8
  • S H. Aghajanshakeri
    • 8
  • S. Javaherypour
    • 8
  • R. Shafiee
    • 4
  • R. Emrani Bidi
    • 9
  1. 1.Department of Pathology, Faculty of Veterinary MedicineTehran UniversityTehranIran
  2. 2.Department of Pathobiology, Susangerd BranchIslamic Azad UniversitySusangerdIran
  3. 3.Department of Animal Science, College of AgricultureIslamic Azad UniversityKashmarIran
  4. 4.Graduate, Faculty of Veterinary MedicineTehran UniversityTehranIran
  5. 5.Faculty of Veterinary MedicineGraduate student of Islamic Azad University of GarmsarGarmsarIran
  6. 6.Science and Research Branch of TehranSmall Animal Internal Medicine Resident of Islamic Azad UniversityTehranIran
  7. 7.Faculty of Para Veterinary MedicineIlam UniversityIlamIran
  8. 8.Student of Veterinary Medicine, Faculty of Veterinary MedicineUrmia UniversityUrmiaIran
  9. 9.EA1274 (M2S) laboratory, Department of PharmacyUniversity of Rennes 1RennesFrance

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