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Tumor Biology

, Volume 35, Issue 6, pp 5493–5500 | Cite as

RETRACTED ARTICLE: Canine transmissible venereal tumor and seminoma: a cytohistopathology and chemotherapy study of tumors in the growth phase and during regression after chemotherapy

  • J. Javanbakht
  • B. Pedram
  • M. R. Taheriyan
  • F. Khadivar
  • S. H. Hosseini
  • F. S. Abdi
  • E. Hosseini
  • M. Moloudizargari
  • S H. Aghajanshakeri
  • S. Javaherypour
  • R. Shafiee
  • R. Emrani Bidi
Research Article

Abstract

In this study, 12 dogs affected by canine transmissible venereal tumor (CTVT) and testicular seminoma tumor were studied retrospectively. The cytological sample was smeared onto a glass slide and either air-dried for May-Grünwald-stain, and masses were surgically removed. The tumors were grossly examined, and sections of 4-μm thick were obtained from each sample and stained with H&E. For chemotherapy, vincristine sulfate was administered weekly as an infusion over 3 min via the cephalic vein at a dose of 0.025 mg/kg after diluting with physiological saline to a total amount of 10 ml. If no remission was observed after 8 weeks, chemotherapy was continued with weekly doxorubicin infusion at a dose of 1 mg/kg. All the tumor samples were divided into four cytohistopathologic groups, namely: multilobular (six cases), papillary (two cases), pedunculated (two cases), and tubular (two cases of seminoma). The most frequently represented tumor type was multilobular (6/10, 60 %) followed by pedunculated (2/10, 20 %), papillary (2/10, 20 %), and tubular (two cases of seminoma, 100 %). Cytological smears from eight tumors in regression after chemotherapy were poorly cellular, and many cells were fragmented. In two progressive tumors, there was an average of 1,406 ± 972 CTVT 200 cells/μl or 96 · 71 % of total cells counted. Thus, tumor cells represented 96 · 71 % of total cells within the biopsy specimens and the leukocytes 4 · 29 % (leukocyte, tumor cell ratio = 0 · 062 ± 0 · 031). In eight regressive tumors, there was an average of 1,245 ± 1,032 CTVT 200 cells/μl or 97 · 31 % of total cells counted. Thus, tumor cells represented 97 · 31 % of total cells and leukocytes 2 · 69 % (leukocyte, tumor cell ratio = 0 · 071 ± 0 · 174). Our data suggested that combination treatment with vincristine and doxorubicin in the future could be an excellent therapeutic alternative for the treatment of TVT for probably reducing the resistance to vincristine, and also, treatment success could easily be followed by the cytological changes.

Keywords

Chemotherapy TVT Seminoma Cytology Pathology Canine 

Notes

Acknowledgments

The authors thank Dr. Saeid Fathi and Dr. Mehdi Aghamohammad Hassan, Faculty of Veterinary Medicine, Tehran University, Iran, for their help with this manuscript.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • J. Javanbakht
    • 1
  • B. Pedram
    • 2
  • M. R. Taheriyan
    • 3
  • F. Khadivar
    • 4
  • S. H. Hosseini
    • 5
  • F. S. Abdi
    • 6
  • E. Hosseini
    • 7
  • M. Moloudizargari
    • 8
  • S H. Aghajanshakeri
    • 8
  • S. Javaherypour
    • 8
  • R. Shafiee
    • 4
  • R. Emrani Bidi
    • 9
  1. 1.Department of Pathology, Faculty of Veterinary MedicineTehran UniversityTehranIran
  2. 2.Department of Pathobiology, Susangerd BranchIslamic Azad UniversitySusangerdIran
  3. 3.Department of Animal Science, College of AgricultureIslamic Azad UniversityKashmarIran
  4. 4.Graduate, Faculty of Veterinary MedicineTehran UniversityTehranIran
  5. 5.Faculty of Veterinary MedicineGraduate student of Islamic Azad University of GarmsarGarmsarIran
  6. 6.Science and Research Branch of TehranSmall Animal Internal Medicine Resident of Islamic Azad UniversityTehranIran
  7. 7.Faculty of Para Veterinary MedicineIlam UniversityIlamIran
  8. 8.Student of Veterinary Medicine, Faculty of Veterinary MedicineUrmia UniversityUrmiaIran
  9. 9.EA1274 (M2S) laboratory, Department of PharmacyUniversity of Rennes 1RennesFrance

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