Tumor Biology

, Volume 35, Issue 6, pp 5481–5486 | Cite as

Clinical implication of TMPRSS4 expression in human gallbladder cancer

  • Xiao-Yang Wu
  • Li Zhang
  • Ke-Ming Zhang
  • Ming-Hua Zhang
  • Ting-Yan Ruan
  • Chao-Ying Liu
  • Jun-Ying Xu
Research Article


Altered expression of transmembrane protease/serine 4 (TMPRSS4) is observed in various types of human cancers. However, the clinical significance of TMPRSS4 expression in gallbladder cancer (GBC) remains largely unknown. The present study aims to explore the clinicopathological significance and prognostic value of TMPRSS4 in GBC. The levels of TMPRSS4 mRNA and protein in GBC tissues and adjacent noncancerous tissues were evaluated by quantitative reverse-transcriptase polymerase chain reaction and immunohistochemistry. To investigate the correlations between TMPRSS4 and the clinicopathological features of GBC, the expression of TMPRSS4 in 97 patients with GBC were detected by immunohistochemistry. The correlation of TMPRSS4 expression with patients’ survival rate was assessed by Kaplan–Meier and Cox regression. Our results showed that the expression levels of TMPRSS4 mRNA and protein in GBC tissues were both significantly higher than those in adjacent noncancerous tissues. Immunohistochemical staining revealed that high TMPRSS4 expression was closely correlated with tumor size (P = 0.032), histological grade (P = 0.002), pathologic T stage (P = 0.005), clinical stage (P = 0.013), and lymph node metastasis (P = 0.003). Moreover, the results of Kaplan–Meier analysis indicated that a high expression level of TMPRSS4 resulted in a significantly poor prognosis of GBC patients. Multivariate analysis showed that the status of TMPRSS4 expression was an independent prognostic factor for GBC patients. Our results showed that TMPRSS4 plays a key role in GBC and therefore may provide an opportunity for developing a novel therapeutic target as well as a prognostic marker in GBC.


TMPRSS4 Immunohistochemistry qRT-PCR Prognosis 


Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Xiao-Yang Wu
    • 1
    • 2
  • Li Zhang
    • 3
  • Ke-Ming Zhang
    • 4
  • Ming-Hua Zhang
    • 5
  • Ting-Yan Ruan
    • 6
  • Chao-Ying Liu
    • 6
  • Jun-Ying Xu
    • 6
  1. 1.Department of Cardiovascular SurgeryThe First Affiliated Hospital of Soochow UniversitySuzhouChina
  2. 2.Department of General SurgeryKunshan First People’s Hospital Affiliated to Jiangsu UniversityKunshanChina
  3. 3.Department of GastroenterologyKunshan First People’s Hospital Affiliated to Jiangsu UniversityKunshanChina
  4. 4.Department of Minimally Invasive SurgeryWuxi People’s Hospital Affiliated to Nanjing Medical UniversityWuxiChina
  5. 5.Department of Hepatobiliary SurgeryKunshan First People’s Hospital Affiliated to Jiangsu UniversityKunshanChina
  6. 6.Department of Medical OncologyWuxi People’s Hospital Affiliated to Nanjing Medical UniversityWuxiChina

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