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Tumor Biology

, Volume 35, Issue 5, pp 4821–4830 | Cite as

Correlation between genetic polymorphisms within IL-1B and TLR4 genes and cancer risk in a Russian population: a case-control study

  • Anton G. Kutikhin
  • Arseniy E. Yuzhalin
  • Alexey N. Volkov
  • Alexey S. Zhivotovskiy
  • Elena B. Brusina
Research Article

Abstract

In the last decade, a growing interest has been devoted to the evaluation of the impact of single nucleotide polymorphisms (SNP) on cancer risk. According to the results of multiple studies, among the genes that have a considerable influence on cancer risk are those encoding pattern recognition receptors, cytokines, and antioxidant defense enzymes. Nonetheless, the effect of numerous SNPs within these genes on cancer risk has been scarcely investigated. A case-control study of 401 cases and 300 sex- and age-matched controls was performed in order to explore the role of IL1B_1473G/C (rs1143623), SOD1_7958A/G (rs4998557), TLR4_1196C/T (rs4986791), IL10_1082A/G (rs1800896), IL17A_197G/A (rs2275913), and TLR4_896A/G (rs4986790) polymorphisms in the susceptibility to colorectal cancer (n = 244), gastric carcinoma (n = 72), and ovarian cancer (n = 85). The analysis revealed a significant relationship between the presence of heterozygous genotypes for IL1B_1473G/C and TLR4_896A/G polymorphisms and higher risk of rectal cancer (codominant model, OR = 1.67; 95 % CI, 1.06–2.63; p = 0.048 and OR = 2.25; 95 % CI, 1.26–4.02; p = 0.014, respectively). In addition, the variant G/G genotype of the IL10_1082A/G SNP was associated with a 2.5-fold increase in ovarian cancer risk with a borderline significance (codominant model, OR = 2.45; 95 % CI, 1.14–5.25; p = 0.069). Similarly, the carriers of the C/T genotype for the TLR4_1196C/T polymorphism were more susceptible to rectal cancer with a borderline significance (codominant model, OR = 1.42; 95 % CI, 0.80–2.51 p = 0.06). No statistically significant associations were found when stratifying the sample by subgroups of age, sex, and clinicopathological characteristics. Finally, we observed six combinations of haplotypes for the examined SNPs, each of which either profoundly increased or decreased cancer risk. The results from our study provided evidence that IL1B_1473G/C and TLR4_896A/G SNPs are implicated in rectal cancer development in a Russian population. Further research should be addressed to clarify the role of the abovementioned polymorphisms in cancer etiology.

Keywords

Single nucleotide polymorphism Interleukin Toll-like receptor Superoxide dismutase Cancer 

Notes

Acknowledgments

The authors wish to acknowledge the assistance and support of Yuri A. Magarill, Yakov M. Kolesnikov, Asiya D. Enikeeva, Tamara N. Agafonova, Olga A. Kozlova, Yuila A. Popova, Pavel N. Arkharov, Andrey S. Sukhikh, Grigory V. Vavin, Sergey F. Zinchuk, and Jeanne V. Turbikova. The authors are also grateful to the staff of the Kemerovo Regional Clinical Oncological Dispensary involved in the study. Special thanks go to Dr. Su Yin (Esther) Lim for careful proofreading and helpful critiques of the manuscript. This research was supported by Bortnik Fund (UMNIK), commercial contracts with the Department of Health of the Population of Kemerovo Region and Kemerovo State Medical Academy President Grant.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Anton G. Kutikhin
    • 1
    • 2
    • 3
  • Arseniy E. Yuzhalin
    • 4
  • Alexey N. Volkov
    • 3
    • 6
  • Alexey S. Zhivotovskiy
    • 5
  • Elena B. Brusina
    • 2
  1. 1.Research Institute for Complex Issues of Cardiovascular Diseases under the Siberian Branch of the Russian Academy of Medical SciencesKemerovoRussia
  2. 2.Department of EpidemiologyKemerovo State Medical AcademyKemerovoRussia
  3. 3.Central Research LaboratoryKemerovo State Medical AcademyKemerovoRussia
  4. 4.Department of Oncology, Gray Institute for Radiation Oncology and BiologyUniversity of OxfordOxfordUK
  5. 5.Kemerovo Regional Clinical Oncological DispensaryKemerovoRussia
  6. 6.Kemerovo State UniversityKemerovoRussia

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