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Tumor Biology

, Volume 35, Issue 5, pp 4551–4559 | Cite as

Relationship of immunohistochemical biomarker expression and lymph node involvement in patients undergoing surgical treatment of NSCLC with long-term follow-up

  • Ana María Gómez
  • Jose Ramón Jarabo Sarceda
  • Jose Antonio L. García-Asenjo
  • Cristina Fernandez
  • Susana Hernandez
  • Julian Sanz
  • Elena Fernandez
  • Joaquin Calatayud
  • Antonio Torres
  • Florentino Hernando
Research Article

Abstract

We try to identify the relationship between immunohistochemical marker expression and lymph node involvement in a cohort of 282 patients followed for 5 years after curative resection for NSCLC. In 189 patients (67 %), lymph nodes were unaffected while 93 patients (33 %) showed nodal involvement. The expression of 15 molecular markers was determined from each patient by tissue-array immunohistochemistry. Univariate analysis indicated significantly higher expression of E-cadherin, γ-catenin, p27, and p53 in patients with lymph node involvement. In those with unaffected nodes, p16 and Rb were expressed. E-cadherin expression was related to a 50 % mortality reduction in patients with node involvement (hazard ratio (HR) 0.5; p = 0.017). c-erbB-2 expression was correlated with a 3.4-fold increase in mortality compared to patients without expression of this marker in subjects without node involvement (HR 3.41; p = 0.017). Multivariate analysis identified c-erbB-2 (HR 2.22; p = 0.089) and p27 (HR 1.44; p = 0.019) as prognostics of mortality while Rb (HR 0.74) indicated a good prognosis. The expression of proteins encoded by oncogenes and tumor suppressor genes was different according to lymph node involvement. The increased mortality related to c-erbB-2 expression in patients with unaffected lymph nodes would suggests a need for adjuvant treatment.

Keywords

Non-small cell lung cancer Gene expression Lymph nodes Tissue array Prognosis c-erb-2 

Notes

Funding source

Fundación Mutua Madrileña.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Ana María Gómez
    • 1
  • Jose Ramón Jarabo Sarceda
    • 1
  • Jose Antonio L. García-Asenjo
    • 2
  • Cristina Fernandez
    • 3
  • Susana Hernandez
    • 4
  • Julian Sanz
    • 4
  • Elena Fernandez
    • 1
  • Joaquin Calatayud
    • 5
  • Antonio Torres
    • 6
  • Florentino Hernando
    • 1
  1. 1.Thoracic Surgery Department, Hospital Clínico San CarlosInstituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)MadridSpain
  2. 2.Department of PathologyHospital Universitario Príncipe de AsturiasAlcalá de HenaresSpain
  3. 3.Department of Epidemiology, Hospital Clínico San CarlosInstituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)MadridSpain
  4. 4.Department of Pathology, Hospital Clínico San CarlosInstituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)MadridSpain
  5. 5.Thoracic Surgery Department, Hospital Clínico San CarlosInstituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)MadridSpain
  6. 6.General Surgery Department, Hospital Clínico San CarlosInstituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)MadridSpain

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