Tumor Biology

, Volume 35, Issue 4, pp 3517–3524 | Cite as

Anticancer effects of deproteinized asparagus polysaccharide on hepatocellular carcinoma in vitro and in vivo

  • Jianfeng Xiang
  • Yanjie Xiang
  • Shengming Lin
  • Dongwei Xin
  • Xiaoyu Liu
  • Lingling Weng
  • Tao ChenEmail author
  • Minguang ZhangEmail author
Research Article


Hepatocellular carcinoma (HCC) is one of the most aggressive malignancies in the world whose chemoprevention became increasingly important in HCC treatment. Although the anticancer effects of asparagus constituents have been investigated in several cancers, its effects on hepatocellular carcinoma have not been fully studied. In this study, we investigated the anticancer effects of the deproteinized asparagus polysaccharide on the hepatocellular carcinoma cells using the in vitro and in vivo experimental model. Our data showed that deproteinized asparagus polysaccharide might act as an effective inhibitor on cell growth in vitro and in vivo and exert potent selective cytotoxicity against human hepatocellular carcinoma Hep3B and HepG2 cells. Further study showed that it could potently induce cell apoptosis and G2/M cell cycle arrest in the more sensitive Hep3B and HepG2 cell lines. Moreover, deproteinized asparagus polysaccharide potentiated the effects of mitomycin both in vitro and in vivo. Mechanistic studies revealed that deproteinized asparagus polysaccharide might exert its activity through an apoptosis-associated pathway by modulating the expression of Bax, Bcl-2, and caspase-3. In conclusion, deproteinized asparagus polysaccharide exhibited significant anticancer activity against hepatocellular carcinoma cells and could sensitize the tumoricidal effects of mitomycin, indicating that it is a potential therapeutic agent (or chemosensitizer) for liver cancer therapy.


Asparagus polysaccharide Hepatocellular carcinoma Apoptosis Cell cycle arrest 



This work was supported by the grant from National Natural Science Foundation of China (30973823), the grant of Shanghai City Population and Family Planning Commission of Science and Technology Development Fund Project (2010JG04), the special grant from the China Postdoctoral Science Foundation (2013 T60791), and the Key Laboratory Construction grant (SW201110010) from Science, Industry, Trade, and Information Technology Commission of Shenzhen Municipality.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Jianfeng Xiang
    • 1
  • Yanjie Xiang
    • 2
  • Shengming Lin
    • 3
  • Dongwei Xin
    • 3
  • Xiaoyu Liu
    • 4
  • Lingling Weng
    • 3
  • Tao Chen
    • 4
    Email author
  • Minguang Zhang
    • 3
    Email author
  1. 1.Department of Radiology, Shanghai Sixth People’s HospitalShanghai JiaoTong UniversityShanghaiPeople’s Republic of China
  2. 2.Department of Obstetrics and Gynecology, Renji Hospital, School of MedicineShanghai JiaoTong UniversityShanghaiPeople’s Republic of China
  3. 3.Department of Radiology, Affiliated Hospital of Traditional Chinese MedicineShanghai University of Traditional Chinese MedicineShanghaiPeople’s Republic of China
  4. 4.School of MedicineShenzhen UniversityShenzhenPeople’s Republic of China

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