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Tumor Biology

, Volume 35, Issue 4, pp 3487–3494 | Cite as

MiR-495 regulates proliferation and migration in NSCLC by targeting MTA3

  • Heying Chu
  • Xudong Chen
  • Huaqi Wang
  • Yuwen Du
  • Yuanyuan Wang
  • Wenqiao Zang
  • Ping Li
  • Juan Li
  • Jingxia Chang
  • Guoqiang Zhao
  • Guojun Zhang
Research Article

Abstract

Our previous studies have showed that metastasis-associated protein 3 (MTA 3) is overexpressed in non-small cell lung cancer (NSCLC) tissue, and increased MTA3 mRNA levels is a risk factor of lymph node metastasis. Using bioinformatics analyses, we found that MTA3 was a potential target of miR-495. However, the pathophysiological role of miR-495 and its relevance to the growth and development of NSCLC have yet to be investigated. The purpose of this study was to elucidate the molecular mechanisms by which miR-495 acts as a tumor suppressor in NSCLC. qRT-PCR data showed significant downregulation of miR-495 in 56 NSCLC tissue samples and 5 lung cancer cell lines, compared with their adjacent normal tissue; furthermore, western blotting analysis revealed MTA3 protein was overexpressed in the tumor samples compared with the matched adjacent normal tissue. MiR-495 was shown to not only inhibit the proliferation of lung cancer cells (A549 and Calu-3) but also to inhibit cell migration in vitro. Using western blotting and luciferase assays, MTA3 was identified as a target of miR-495. These findings suggest the importance of miR-495 targeting of MTA3 in the regulation of lung cancer growth and migration.

Keywords

miR-495 MTA3 NSCLC Proliferation Migration 

Notes

Acknowledgments

This study was supported by Science and Technology Commission of Henan Province of China (no. 122102310552).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Heying Chu
    • 1
  • Xudong Chen
    • 2
  • Huaqi Wang
    • 1
  • Yuwen Du
    • 3
  • Yuanyuan Wang
    • 3
  • Wenqiao Zang
    • 3
  • Ping Li
    • 1
  • Juan Li
    • 1
  • Jingxia Chang
    • 1
  • Guoqiang Zhao
    • 3
  • Guojun Zhang
    • 1
  1. 1.Department of Respiratory MedicineThe First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina
  2. 2.Department of Histology and EmbryologyLuohe Medical CollegeLuoheChina
  3. 3.College of Basic Medical SciencesZhengzhou UniversityZhengzhouChina

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