Tumor Biology

, Volume 35, Issue 3, pp 2549–2559 | Cite as

Induction of apoptosis by total flavonoids from Scutellaria barbata D. Don in human hepatocarcinoma MHCC97-H cells via the mitochondrial pathway

  • Jie Gao
  • Wang-Feng Lu
  • Zhi-Jun Dai
  • Shuai Lin
  • Yang Zhao
  • Sha Li
  • Nuan-Nuan Zhao
  • Xi-Jing Wang
  • Hua-Feng Kang
  • Xiao-Bin Ma
  • Wang-Gang Zhang
Research Article

Abstract

Scutellaria barbata D. Don, a traditional Chinese medicine, reportedly possesses antitumor activity against a variety of tumors. In the present study, we investigated the cytotoxic effect of total flavonoids from S. barbata (TF-SB) on human hepatocarcinoma cells and the underlying molecular mechanisms regarding the effect were explored. TF-SB treatment significantly reduced the cell viability of human HCC MHCC97-H cells in a dose-dependent manner. Further flow cytometric analysis showed that the apoptosis rate of MHCC97-H cells increased and the mitochondrial membrane potential (∆ψm) of MHCC97-H cells decreased after TF-SB treatment. DNA ladder showed that TF-SB induced a significant increase in DNA fragmentation in MHCC97-H cells. Reverse transcription PCR and Western blot analysis revealed that the expression levels of Smac, Apaf-1, Cytochrome c, Caspase-9, and Caspase-3 were upregulated in a dose-dependent manner and after treatment with different concentrations of TF-SB for 48 h. These results suggest that TF-SB induces apoptosis in MHCC97-H cells through the mitochondrial pathway.

Keywords

Scutellaria barbata Hepatocarcinoma Appptosis Mitochondrial 

Abbreviations

HCC

Hepatocellular carcinoma

TF-SB

Total flavones of Scutellaria barbatae

∆ψm

Mitochondrial membrane potential

MTT

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide

DMEM

Dulbecco’s modified Eagle’s medium

Smac

Second mitochondria-derived activator of caspase

Apaf-1

Apoptotic protease activating factor

RT-PCR

Reverse transcription polymerase chain reaction

HPLC

High-performance liquid chromatography

PI

Propidium iodide

FCM

Flow cytometry

Notes

Acknowledgments

This study was supported by National Natural Science Foundation of China, no. 81102711; the Fundamental Research Funds for the Central Universities, China, no. xjj2011039; and Sci-Tech Program of Administration of Traditional Chinese Medicine of Shaanxi Province, China, no. 13-JC005.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Jie Gao
    • 1
    • 2
  • Wang-Feng Lu
    • 1
    • 3
  • Zhi-Jun Dai
    • 1
  • Shuai Lin
    • 1
  • Yang Zhao
    • 1
  • Sha Li
    • 4
  • Nuan-Nuan Zhao
    • 4
  • Xi-Jing Wang
    • 1
  • Hua-Feng Kang
    • 1
  • Xiao-Bin Ma
    • 1
  • Wang-Gang Zhang
    • 5
  1. 1.Department of OncologyThe Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’anChina
  2. 2.Department of NephrologyThe Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’anChina
  3. 3.Department of Surgical OncologyShangluo Central HospitalShangluoChina
  4. 4.Department of PharmacologyThe Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’anChina
  5. 5.Department of HematologyThe Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’anChina

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