Tumor Biology

, Volume 35, Issue 3, pp 2047–2051 | Cite as

Autoantibodies against p16 protein-derived peptides may be a potential biomarker for non-small cell lung cancer

  • Cong Zhang
  • Leiguang Ye
  • Songlei Guan
  • Shunzi Jin
  • Weili Wang
  • Shilong Sun
  • Kuang-Hui Lee
  • Jun Wei
  • Baogang Liu
Research Article


Overexpression of tumor-associated antigens (TAAs) has been reported in many types of cancer and may trigger secretion of their autoantibodies. The present work was thus designed to test whether circulating antibody to p16 protein-derived antigens was altered in lung cancer. Two hundred seventy-one patients with non-small cell lung cancer (NSCLC) and 226 control subjects matched in age, gender, and smoking history were recruited in this study. The levels of circulating anti-p16 IgA and IgG antibodies were tested using an enzyme-linked immunosorbent assay (ELISA) developed in-house with linear peptide antigens derived from p16 protein. Student’s t test showed that patients with NSCLC had a significant higher level of anti-p16 IgG antibody than control subjects (t = 2.74, P = 0.0063) but did not have a significant increase in IgA antibody levels (t = 1.92, P = 0.056). The sensitivity against >90 % specificity was 19.7 % for the IgG assay with an inter-assay deviation of 11.6 %, and 10.3 % for the IgA assay with an inter-assay deviation of 14.7 %. Based on a cut-off value determined by the 99th percentile of control IgG levels, the anti-p16 IgG positivity was 6.7 % in patients with NSCLC compared to 0.88 % in control subjects (χ 2 = 10.58, P = 0.001, OR = 7.97, 95 % CI 1.84–34.85). Circulating anti-p16 IgG levels were increased with stages of NSCLC, and patients with stage IV NSCLC had the highest IgG level among all four stages (t = 2.42, P = 0.016, compared with the control group). Pearson correlation analysis showed a significant correlation between circulating levels of IgA and IgG in the patient group (r = −0.2, df = 236, P = 0.0021) but not in the control group (r = −0.1, df = 205, P = 0.146). Circulating IgG antibody to p16 protein may be a potential biomarker with prognostic values for lung cancer.


Autoantibody Biomarker p16 protein Lung cancer Tumor immunity 



We thank patients and healthy volunteers for their support and participation. This work was supported by the Natural Science Foundation of Heilongjiang Province, Harbin, China (grant number D201238), by YingJi Biotechnology & Exploitation Ltd, Shenzhen, China, and by Glory Biomedical Co. Ltd, Taipei, Taiwan.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Cong Zhang
    • 1
  • Leiguang Ye
    • 2
  • Songlei Guan
    • 1
  • Shunzi Jin
    • 1
  • Weili Wang
    • 1
  • Shilong Sun
    • 1
  • Kuang-Hui Lee
    • 3
  • Jun Wei
    • 4
  • Baogang Liu
    • 2
  1. 1.Department of Radiobiology, School of Public HealthJilin UniversityChangchunChina
  2. 2.Department of Pulmonary OncologyThe Third Affiliated Hospital of Harbin Medical UniversityHarbinChina
  3. 3.Pei-Ling Guan-Si HospitalHsinchu CountyTaiwan
  4. 4.Department of Diabetes & Cardiovascular ScienceUniversity of the Highlands & Islands, Centre for Health ScienceInvernessUK

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