Cytokines in osteoblast-conditioned medium promote the migration of breast cancer cells
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Bone is one of the most common metastatic sites for breast cancer. In this study, we observed a promoting effect of osteoblast-conditioned medium (OCM) on the migration of MCF-7, a noninvasive cell line of breast cancer cells. Cytokine antibody array was used to compare the cytokines of OCM with the conditioned medium of non-differentiated osteoblast cells, which consequently revealed factors related to migration, such as IL8, IL6, CSF2 (G-CSF), CSF3 (GM-CSF), and TNFRSF11B (osteoprotegerin). The expression of genes related to migration was also estimated with a PCR array, which showed that 9 genes were upregulated and 26 genes downregulated. Moreover, activated p38, ERK, and AKT pathways were found in the OCM treatment group. This finding indicated the migration ability of breast cancer cells, which move toward the bone depending on the presence of specific cytokines in its surrounding microenvironment.
KeywordsCytokines Osteoblast-conditioned medium (OCM) Breast cancer Bone microenvironment
Differentiated osteoblast-conditioned medium
Undifferentiated osteoblast-conditioned medium
The authors thank Prof. Binqian Shen for the revisions on our paper. This work was supported by the Project of Production, Education and Research of Guangdong Province (2010B090400500) and the 211 Grant of the Ministry of Education of China. The funding agencies had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- 23.Mahabeleshwar GH, Kundu GC. Syk, a protein-tyrosine kinase, suppresses the cell motility and nuclear factor kappa b-mediated secretion of urokinase type plasminogen activator by inhibiting the phosphatidylinositol 3′-kinase activity in breast cancer cells. J Biol Chem. 2003;278:6209–21.PubMedCrossRefGoogle Scholar
- 40.Bronner F, Farach-Carson MC. Inflammatory cytokines and their role in bone metastasis and osteolysis. Bone and Cancer. 2009;5:141–55.Google Scholar
- 43.Santini D, Schiavon G, Vincenzi B, Gaeta L, Pantano F, Russo A, et al. Receptor activator of NF-kB (RANK) expression in primary tumors associates with bone metastasis occurrence in breast cancer patients. PLoS One. 2011;6:1–9.Google Scholar
- 44.Eubank TD, Roberts RD, Khan M, Curry JM, Nuovo GJ, Kuppusamy P, et al. Granulocyte macrophage colony-stimulating factor inhibits breast cancer growth and metastasis by invoking an anti-angiogenic program in tumor-educated macrophages. Cancer Research. 2009;69:2133–40.PubMedCentralPubMedCrossRefGoogle Scholar