Tumor Biology

, Volume 34, Issue 6, pp 3743–3751 | Cite as

Thrombomodulin mediates the progression of epithelial ovarian cancer cells

  • Lu-Min Chen
  • Weu Wang
  • Jen-Chih Lee
  • Feng-Hsiang Chiu
  • Chun-Te Wu
  • Cheng-Jeng Tai
  • Chien-Kai Wang
  • Chen-Jei Tai
  • Ming-Te Huang
  • Yu-Jia Chang
Research Article


Thrombomodulin (TM), a natural anticoagulation factor, maintains circulation homeostasis in endothelial cells. TM has additional roles in modulating inflammation, thrombosis, and carcinogenesis. However, there is little information on the role of TM in the progression and metastasis of ovarian cancer. RNA silencing and cDNA expression vectors were used to manipulate target gene expression in ovarian cancer cells. Cell growth and migration were evaluated by an MTT assay, a wound-healing migration assay, a transwell migration assay, and a biosensor system. In this study, we found that TM silencing may enhance the growth rate of cells. The migratory ability of ovarian cancer cells was enhanced dramatically after TM silencing. TM overexpression in ovarian cells suppressed the proliferation and migration capability. Furthermore, we found that skov-3 cells treated with TM shRNA expressed high levels of fibronectin and vimentin and that the expression of these markers correlated positively with their migratory ability. Our results demonstrate that TM expression may regulate cell growth and migration in ovarian cancer cells. This finding suggests that TM may be a novel prognostic and therapeutic target for ovarian cancer.


Thrombomodulin Ovarian cancer Migration 





Epithelial-mesenchymal transition


Epithelial ovarian cancer



This study was supported by a grant from the Taipei Medical University/Shuang Ho Hospital (100TMU-SHH-08) and grants from National Science Council (NSC101-2314-B-038-030-MY2 and NSC101-2314-B-038-016-MY3).

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Lu-Min Chen
    • 1
    • 2
  • Weu Wang
    • 3
    • 4
  • Jen-Chih Lee
    • 15
  • Feng-Hsiang Chiu
    • 16
  • Chun-Te Wu
    • 5
    • 6
  • Cheng-Jeng Tai
    • 7
    • 8
  • Chien-Kai Wang
    • 7
    • 8
    • 9
    • 10
  • Chen-Jei Tai
    • 9
    • 10
  • Ming-Te Huang
    • 3
    • 4
    • 11
  • Yu-Jia Chang
    • 3
    • 4
    • 12
    • 13
    • 14
  1. 1.Department of Obstetrics and Gynecology, Suzhou BenQ Medical CenterNanjing Medical UniversitySuzhouPeople’s Republic of China
  2. 2.Department of Obstetrics and GynecologyChina Medical University HospitalTaichingRepublic of China
  3. 3.Department of Surgery, College of MedicineTaipei Medical UniversityTaipei 110Republic of China
  4. 4.Department of SurgeryTaipei Medical University HospitalTaipeiRepublic of China
  5. 5.Chang Gung University College of MedicineTaipeiRepublic of China
  6. 6.Department of UrologyChang Gung Memorial Hospital at KeelungKeelungRepublic of China
  7. 7.Division of Hematology and Oncology, Department of Internal MedicineTaipei Medical University HospitalTaipeiRepublic of China
  8. 8.Department of Internal Medicine, School of Medicine, College of MedicineTaipei Medical UniversityTaipeiRepublic of China
  9. 9.Department of Chinese MedicineTaipei Medical University HospitalTaipeiRepublic of China
  10. 10.Department of Obstetrics and Gynecology, School of Medicine, College of MedicineTaipei Medical UniversityTaipeiRepublic of China
  11. 11.Division of General Surgery, Department of Surgery, Shuang Ho HospitalTaipei Medical UniversityTaipeiRepublic of China
  12. 12.Graduate Institute of Clinical Medicine, College of MedicineTaipei Medical UniversityTaipei 110Republic of China
  13. 13.Center of Excellence for Cancer ResearchTaipei Medical UniversityTaipeiRepublic of China
  14. 14.Cancer Research CenterTaipei Medical University HospitalTaipeiRepublic of China
  15. 15.Department of Internal Medicine, Suzhou BenQ Medical CenterNanjing Medical UniversitySuzhouPeople’s Republic of China
  16. 16.Department of Emergency MedicineChang-Gung Memorial Hospital, Linkou BranchLinkouRepublic of China

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