Tumor Biology

, Volume 34, Issue 6, pp 3619–3625 | Cite as

Transcription factor AP1 binds the functional region of the promoter and regulates gene expression of human PPARdelta in LoVo cell

  • Xiaogang Jiang
  • Xudong Yang
  • Yan Han
  • Shemin Lu
Research Article


Peroxisome proliferator-activated receptor δ gene (PPARδ) is correlated with carcinogenesis of colorectal cancer, but the regulation of its gene transcription remains unclear. We herein report that AP1 binds the promoter and regulates PPARδ gene expression. With a luciferase reporter system, we identified a functional promoter region of 30 bp of PPARδ gene by deletion and electrophoretic mobility shift assays (EMSA). Using site-directed mutagenesis and decoy analyses, we demonstrated that AP1 bound the functional transcriptional factor binding site in a region extending from −176 to −73 of the PPARδ promoter, which was confirmed using EMSA and supershift assays. Consequently, inhibition of the AP1 binding site led to decreased PPARδ mRNA. Our study demonstrated that AP1 is the transcriptional factor that contributes to PPARδ expression in LoVo cells.


Peroxisome proliferator-activated receptor (PPAR) AP1 (nuclear transcription factor activator protein-1) Electrophoresis mobility shift analysis (EMSA) Supershift Chip LoVo cells 


Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Xiaogang Jiang
    • 1
  • Xudong Yang
    • 1
  • Yan Han
    • 1
  • Shemin Lu
    • 1
  1. 1.Department of Genetics and Molecular BiologyXi’an Jiaotong University College of MedicineXi’anPeople’s Republic of China

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