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Tumor Biology

, Volume 34, Issue 5, pp 2951–2959 | Cite as

Effects of CXCR4 gene silencing by lentivirus shRNA on proliferation of the EC9706 human esophageal carcinoma cell line

  • Dao-feng Wang
  • Ning Lou
  • Miao-zhen Qiu
  • Yong-bin Lin
  • Ying Liang
Research Article

Abstract

CXCL12/CXCR4 has been studied as an important biomarker for many human malignancies, but studies are limited for esophageal squamous cell carcinoma (ESCC). In this study, an effective RNAi sequence targeting the CXCR4 gene was selected, a lentiviral shRNA vector was constructed to specifically silence CXCR4 expression in the EC9706 ESCC cell line, and the effects of CXCR4 silencing on cell growth in vitro and tumour growth in nude mice were then evaluated. The expression of CXCR4 in EC9706 was significantly downregulated after transfection with a lentiviral shRNA vector. The expression of the apoptosis-related gene Bcl-2 was decreased. In addition, after CXCR4 inhibition, cell growth was considerably inhibited, increased apoptosis in the EC9706 cells was found, the G0/G1 percentage was significantly increased, and the number of cells in S phase was reduced. Moreover, tumour growth in nude mice was inhibited. In conclusion, the downregulation of CXCR4 expression by transfection with a lentiviral shRNA vector in ESCC cells could inhibit tumour proliferation. Our data may provide an avenue for finding new ESCC treatments.

Keywords

CXCL12 CXCR4 Esophageal squamous cell carcinoma RNA interference Proliferation 

Notes

Acknowledgments

We gratefully thank the staff members in the Department of Medical Oncology and Thoracic Surgery Oncology at Sun Yat-sen University Cancer Center for their suggestion and assistance.

Conflicts of interest

None

Funding

This work was supported by a grant from the Natural Science Foundation of the Guangdong Province of China (S2011040004502).

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Dao-feng Wang
    • 1
    • 2
  • Ning Lou
    • 1
    • 2
  • Miao-zhen Qiu
    • 1
    • 3
  • Yong-bin Lin
    • 1
    • 4
  • Ying Liang
    • 1
    • 3
  1. 1.State Key Laboratory of Oncology in South ChinaGuangzhouPeople’s Republic of China
  2. 2.Department of ICUSun Yat-Sen University Cancer CenterGuangzhouPeople’s Republic of China
  3. 3.Department of Medical OncologySun Yat-Sen University Cancer CenterGuangzhouPeople’s Republic of China
  4. 4.Department of Thoracic SurgerySun Yat-Sen University Cancer CenterGuangzhouPeople’s Republic of China

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