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Tumor Biology

, Volume 34, Issue 5, pp 2691–2696 | Cite as

Cytogenetic characterization of an N-butyl-N-(4-hydroxybutyl) nitrosamine-induced mouse papillary urothelial carcinoma

  • Regina Arantes-Rodrigues
  • Rosário Pinto-Leite
  • Rui Gil da Costa
  • Aura Colaço
  • Carlos Lopes
  • Paula Oliveira
Research Article

Abstract

Chemically-induced urinary bladder cancer in rodents has long been used as a reliable model to study the biopathology of urinary bladder neoplasia and to develop therapeutic strategies against human tumors. Knowledge of the genetic basis underlying carcinogenesis would greatly enhance usability and usefulness of this model for the purposes of comparative pathology. However, little is known about the cytogenetic characteristics of rodent urinary bladder tumors. Accordingly, pathological and negative control specimens were collected for cytogenetic evaluation, from an ongoing mouse urinary bladder N-butyl-N-(4-hydroxybutyl) nitrosamine-induced carcinogenesis study. Histopathological analysis characterized the pathological sample as a papillary urothelial carcinoma. Conventional cytogenetic analysis revealed the presence of 66.3 % tetraploid cells. Fluorescent in situ hybridization using chromosome paint probes allowed the detection of a reciprocal translocation involving chromosomes 4 and 14 (containing the murine homologues to human p16 and retinoblastoma tumor-suppressor genes) in 42 % of tetraploid cells. The control sample showed no histological or cytogenetic changes. CDKN2A and RB1 loss of heterozygosity is associated with human early and advanced urinary bladder cancer, respectively. Thus, the present data paves the way for further studies concerning the molecular mechanisms of urinary bladder carcinogenesis.

Keywords

Urinary bladder cancer Mouse N-butyl-N-(4-hydroxybutyl) nitrosamine Papillary urothelial carcinoma Fluorescent in situ hybridization 

Notes

Acknowledgments

This study was supported by a grant from the Fundação para a Ciência e Tecnologia, Ministério da Educação, Portugal, grant number SFRH/BD/47612/2008 and by FCT Pest-OE/AGR/UI0772/2011 unity.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Regina Arantes-Rodrigues
    • 1
    • 2
  • Rosário Pinto-Leite
    • 3
  • Rui Gil da Costa
    • 4
  • Aura Colaço
    • 5
  • Carlos Lopes
    • 4
  • Paula Oliveira
    • 1
    • 2
  1. 1.Department of Veterinary Sciences, ECAVUniversity of Trás-os-Montes and Alto DouroVila RealPortugal
  2. 2.Center for the Study of Animal Sciences (CECA), ECAVUniversity of Trás-os-Montes and Alto DouroVila RealPortugal
  3. 3.Genetic Service, Cytogenetic LaboratoryHospital Center of Trás-os-Montes and Alto DouroVila RealPortugal
  4. 4.Pathology and Molecular Immunology, Abel Salazar Institute of Biomedical SciencesOporto UniversityPortoPortugal
  5. 5.Department of Veterinary Sciences, CECAVUniversity of Trás-os-Montes and Alto DouroVila RealPortugal

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