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Prognostication of OCT4 isoform expression in prostate cancer

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Tumor Biology

An Erratum to this article was published on 01 December 2013

Abstract

Cancer stem cells (CSCs) refer to a subset of tumor cells that self-renew and affect tumor heterogeneity. This model has attracted considerable interest in recent years due to its implications in the prognosis and clinical management of cancer because CSCs mediate the occurrence, growth, and recurrence of tumors. OCT4 is central to embryonic stem cell self-renewal and differentiation into specific lineages and encodes two chief isoforms that are generated by alternative splicing—OCT4A and OCT4B. Their function in prostate cancer (PCa) is unknown. The prognostic function of OCT4 isoforms in PCa samples was examined by immunohistochemistry (IHC) and sensitivity and specificity of the antibodies used were evaluated by molecular biology techniques. Biochemical and pathological data and specimens from 193 patients with PCa were evaluated retrospectively. IHC, western blot, immunofluorescence, and automated image analysis were also performed. IHC was performed on a tissue microarray, and western blot and immunofluorescence were performed using the PCa cell line DU-145. IHC expression of OCT4 isoforms correlated with biochemical and pathological parameters, particularly biochemical recurrence-free survival (BCRFS). Patients with higher levels of OCT4B had lower Gleason scores and decreased likelihood of experiencing biochemical recurrence (BR). OCT4A+ OCT4B− patients had the shortest BCRFS, and positivity for OCT4B expression was an independent prognostic factor for BCRFS in the multivariate analysis. We conclude that the expression of OCT4B is a strong marker of good prognosis, and its presence is associated with a decreased likelihood of BR. Thus, OCT4B might represent a powerful clinical prognostic biomarker for PCa patients.

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Acknowledgments

We acknowledge Fundação de Amparo à Pesquisa do Estado de São Paulo.

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Authors and Affiliations

  1. Department of Anatomic Pathology, A.C. Camargo Cancer Hospital, Rua Professor Antônio Prudente 211, Liberdade, Sao Paulo, SP, 01509-900, Brazil

    Marina França de Resende, Samantha Vieira, Juliano Jampietro, José Vassallo, Fernando Augusto Soares & Rafael Malagoli Rocha

  2. Department of Clinical Oncology, A.C. Camargo Cancer Hospital, Sao Paulo, Brazil

    Ludmilla Thomé Domingos Chinen

  3. Department of Urology, A.C. Camargo Cancer Hospital, Sao Paulo, Brazil

    Francisco Paulo da Fonseca & Gustavo Cardoso Guimarães

  4. Laboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil

    Luciene Cristina Campos

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  1. Marina França de Resende
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  10. Rafael Malagoli Rocha
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Correspondence to Rafael Malagoli Rocha.

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de Resende, M.F., Chinen, L.T.D., Vieira, S. et al. Prognostication of OCT4 isoform expression in prostate cancer. Tumor Biol. 34, 2665–2673 (2013). https://doi.org/10.1007/s13277-013-0817-9

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  • DOI: https://doi.org/10.1007/s13277-013-0817-9

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