Upregulated hPuf-A promotes breast cancer tumorigenesis
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hPuf-A is a member of RNA-binding PUF family that regulates mRNA translation. Redistribution of hPuf-A from the nucleolus to the nucleoplasm upon genotoxic stress modulates the poly(ADP-ribosyl)ation activity of PARP-1. Here, we report a novel function of hPuf-A involved in promoting breast cancer progression. Immunohistochemical studies showed higher expression levels of hPuf-A in stage I, II, III, and IV breast cancer specimens in contrast with those of hPuf-A in ductal carcinoma in situ. The presence of hPuf-A is highly associated with colony formation capacities in breast cancer T47D and MDA-MB-231 cells. Xenograft growth of hPuf-A-silenced and hPuf-A overexpressing MDA-MB-231 cells in nude mice was substantially in concert with colony formation capacities. This promoting effect of hPuf-A in tumorigenesis might be correlated with the regulation of its associated mRNAs, such as RbAp48 and DDX3. Collectively, hPuf-A may have diagnostic values in breast cancer progression.
KeywordshPuf-A Puf Breast cancer DDX3 RbAp48
We are grateful to the staffs of the TC5 Bio-Image Tools, College of Life Science, National Taiwan University, for the microscopic assistance. We thank the technical services provided by Dr. Sheng-Wei Lin at IBC, Academia Sinica. This work was partly supported by the National Science Council of Taiwan (NSC 101-2311-B-002-008) and National Taiwan University (10R70602B3) to MSC.
Conflicts of interest