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Tumor Biology

, Volume 34, Issue 5, pp 2557–2564 | Cite as

Upregulated hPuf-A promotes breast cancer tumorigenesis

  • Chi-Chen Fan
  • Liek-Yeow Lee
  • Ming-Ying Yu
  • Chi-Yuan Tzen
  • Chi Chou
  • Mau-Sun Chang
Research Article

Abstract

hPuf-A is a member of RNA-binding PUF family that regulates mRNA translation. Redistribution of hPuf-A from the nucleolus to the nucleoplasm upon genotoxic stress modulates the poly(ADP-ribosyl)ation activity of PARP-1. Here, we report a novel function of hPuf-A involved in promoting breast cancer progression. Immunohistochemical studies showed higher expression levels of hPuf-A in stage I, II, III, and IV breast cancer specimens in contrast with those of hPuf-A in ductal carcinoma in situ. The presence of hPuf-A is highly associated with colony formation capacities in breast cancer T47D and MDA-MB-231 cells. Xenograft growth of hPuf-A-silenced and hPuf-A overexpressing MDA-MB-231 cells in nude mice was substantially in concert with colony formation capacities. This promoting effect of hPuf-A in tumorigenesis might be correlated with the regulation of its associated mRNAs, such as RbAp48 and DDX3. Collectively, hPuf-A may have diagnostic values in breast cancer progression.

Keywords

hPuf-A Puf Breast cancer DDX3 RbAp48 

Notes

Acknowledgments

We are grateful to the staffs of the TC5 Bio-Image Tools, College of Life Science, National Taiwan University, for the microscopic assistance. We thank the technical services provided by Dr. Sheng-Wei Lin at IBC, Academia Sinica. This work was partly supported by the National Science Council of Taiwan (NSC 101-2311-B-002-008) and National Taiwan University (10R70602B3) to MSC.

Conflicts of interest

None

Supplementary material

13277_2013_801_MOESM1_ESM.doc (1.7 mb)
ESM 1 (DOC 1706 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Chi-Chen Fan
    • 1
  • Liek-Yeow Lee
    • 2
  • Ming-Ying Yu
    • 2
  • Chi-Yuan Tzen
    • 3
  • Chi Chou
    • 3
  • Mau-Sun Chang
    • 2
    • 4
  1. 1.Department of PhysiologyMackay Memorial HospitalTaipeiTaiwan
  2. 2.Institute of Biochemical Sciences, College of Life ScienceNational Taiwan UniversityTaipeiTaiwan
  3. 3.Department of PathologyMackay Memorial HospitalTaipeiTaiwan
  4. 4.Institute of Biological ChemistryAcademia SinicaTaipeiTaiwan

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