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Tumor Biology

, Volume 34, Issue 4, pp 2419–2426 | Cite as

Adenovirus arming human IL-24 inhibits neuroblastoma cell proliferation in vitro and xenograft tumor growth in vivo

  • Baobiao Zhuo
  • Rong Wang
  • Yiyu Yin
  • Hongwei Zhang
  • Tongsheng Ma
  • Fengli Liu
  • Hui Cao
  • Yingchun Shi
Research Article

Abstract

Data have increasingly shown that interlukin-24 (IL-24) has growth suppression activity and can induce apoptosis in a broad spectrum of tumor cells. However, the therapeutic effect of IL-24 on human neuroblastoma has rarely been explored. In this study, we used a human neuroblastoma cell line (SH-SY5Y) to reveal the effect of adenovirus-mediated IL-24 (Ad-IL24) gene therapy for neuroblastoma. We showed that Ad-IL24 effectively inhibited the proliferation of SH-SY5Y cells in vitro by conspicuously inducing apoptosis. To further explore the molecular mechanism by which Ad-IL24 induced apoptosis in SH-SY5Y tumor cells, we found that Ad-IL24 increased the expression of Bax and promoted the activation of caspase-3, while decreasing Bcl-2 levels. We also demonstrated that Ad-IL24 significantly inhibited tumor growth in vivo in a xenograft neuroblastoma tumor in athymic nude mice. In summary, Ad-IL24 overexpression exerted potent antitumor activity via inducing apoptosis in neuroblastoma cells. Therefore, IL-24 has the potential to serve as an agent for gene therapy in the treatment of neuroblastoma.

Keywords

Neuroblastoma IL-24 Gene therapy Apoptosis 

Notes

Acknowledgments

We are very grateful to Profession Junnian Zheng for providing us with the plasmid carrying the IL-24 cDNA (Laboratory of Biological Cancer Therapy, Xuzhou Medical College).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Baobiao Zhuo
    • 1
  • Rong Wang
    • 2
  • Yiyu Yin
    • 1
  • Hongwei Zhang
    • 1
  • Tongsheng Ma
    • 1
  • Fengli Liu
    • 1
  • Hui Cao
    • 1
  • Yingchun Shi
    • 1
  1. 1.Department of SurgeryXuzhou Children’s HospitalXuzhouChina
  2. 2.Department of UltrasoundThe Affiliated Hospital of Xuzhou Medical CollegeXuzhouChina

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