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Reduced succinate dehydrogenase B expression is associated with growth and de-differentiation of colorectal cancer cells

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Tumor Biology

Abstract

Succinate dehydrogenases (SDH), including SDHA, SDHB, SDHC, and SDHD, form the respiratory complex II in the mitochondria and play an important role in cell growth and homeostasis. In order to evaluate the expression and functional significance of SDH in colorectal cancer, the expression of four SDH subunits was analyzed, and SDHB protein was found to be significantly lower in colorectal cancer tissues. In vitro experiments including cell growth assay, colony formation assay, cell cycle analysis, and nude mouse xenograft of SDHB-transfected colorectal cancer cell line SW620 were performed. Notably, reduced SDHB expression in tumor tissues was associated with tumor de-differentiation, and restoration of SDHB could inhibit the growth of cancer cells both in vitro and in vivo. Furthermore, cDNA microarray of SDHB-transfected cell line showed that most of the differentially expressed genes are related to cell cycle control and cell proliferation. Thus, we conclude that SDHB expression is significantly decreased in human colorectal cancer tissues, and reconstitution of SDHB in colorectal cancer may be a potential therapeutic approach to inhibit aggressiveness of colorectal cancer.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (no. 81172300, 81000883). We are grateful for the support of Medjaden Bioscience Limited.

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Author notes

  1. Shuo Huang

    Present address: Department of Oncology, The First People’s Hospital of Xiangtan City, Xiangtan, Hunan, China

  2. Weiwei Zhou

    Present address: Department of Gastroenterology, The First Affiliated Hospital of South China University, Hengyang, Hunan, China

Authors and Affiliations

  1. Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China

    Decai Zhang, Wei Wang, Nan Li, Shuo Huang, Weiwei Zhou, Yingnan Sun, Xiaoyan Wang, Xiayu Li & Shourong Shen

  2. Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha, Hunan, China

    Decai Zhang, Wei Wang, Bo Xiang, Nan Li, Shuo Huang, Weiwei Zhou, Yingnan Sun, Xiaoyan Wang, Jian Ma, Guiyuan Li, Xiayu Li & Shourong Shen

  3. Cancer Research Institute, Central South University; Key Laboratory of Carcinogenesis, Ministry of Health; Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Changsha, Hunan, China

    Bo Xiang, Jian Ma & Guiyuan Li

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  1. Decai Zhang
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Correspondence to Xiayu Li or Shourong Shen.

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Zhang, D., Wang, W., Xiang, B. et al. Reduced succinate dehydrogenase B expression is associated with growth and de-differentiation of colorectal cancer cells. Tumor Biol. 34, 2337–2347 (2013). https://doi.org/10.1007/s13277-013-0781-4

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  • DOI: https://doi.org/10.1007/s13277-013-0781-4

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