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Overexpression of ECRG4 enhances chemosensitivity to 5-fluorouracil in the human gastric cancer SGC-7901 cell line

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Tumor Biology

Abstract

The aim of this study was to examine the effects of esophageal cancer-related gene 4 (ECRG4) expression levels on chemotherapeutic sensitivity of gastric cancer cells. A SGC-7901 cell system with tetracycline-inducible ECRG4 expression (SGC-7901/ECRG4) was successfully established. ECRG4 mRNA and protein expression levels were detected using quantitative reverse transcription polymerase chain reaction and Western blotting, respectively. Chemosensitivity to 5-fluorouracil (5-FU) was examined by cell proliferation assay and cell apoptosis assay. ECRG4 mRNA and protein expression levels were significantly upregulated in SGC-7901/ECRG4 cells induced with tetracycline. Compared with control cells, the growth inhibition rate of cells with ECRG4 overexpression was significantly increased when treated with 5-FU. Treatment with 5 μmol/l 5-FU resulted in 15.2 % apoptotic cells, whereas such treatment after overexpression of ECRG4 resulted in 44.5 % apoptotic cells. In conclusion, overexpression of ECRG4 enhanced the chemosensitivity of gastric cancer SGC-7901 cells to 5-FU through induction of apoptosis.

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Authors and Affiliations

  1. Department of Pharmacy, Affiliated Hospital of North Sichuan Medical College, No. 63, Wenhua Rd., Nanchong, 637000, China

    Cheng-Ping Jiang, Bi-Hua Wu, Bai-Qiang Wang, Mao-Yong Fu, Ming Yang, Yue Zhou & Fu Liu

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  1. Cheng-Ping Jiang
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  2. Bi-Hua Wu
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  3. Bai-Qiang Wang
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  4. Mao-Yong Fu
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  5. Ming Yang
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  6. Yue Zhou
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  7. Fu Liu
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Correspondence to Bi-Hua Wu.

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Jiang, CP., Wu, BH., Wang, BQ. et al. Overexpression of ECRG4 enhances chemosensitivity to 5-fluorouracil in the human gastric cancer SGC-7901 cell line. Tumor Biol. 34, 2269–2273 (2013). https://doi.org/10.1007/s13277-013-0768-1

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  • DOI: https://doi.org/10.1007/s13277-013-0768-1

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