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Tumor Biology

, Volume 34, Issue 4, pp 2269–2273 | Cite as

Overexpression of ECRG4 enhances chemosensitivity to 5-fluorouracil in the human gastric cancer SGC-7901 cell line

  • Cheng-Ping Jiang
  • Bi-Hua Wu
  • Bai-Qiang Wang
  • Mao-Yong Fu
  • Ming Yang
  • Yue Zhou
  • Fu Liu
Research Article

Abstract

The aim of this study was to examine the effects of esophageal cancer-related gene 4 (ECRG4) expression levels on chemotherapeutic sensitivity of gastric cancer cells. A SGC-7901 cell system with tetracycline-inducible ECRG4 expression (SGC-7901/ECRG4) was successfully established. ECRG4 mRNA and protein expression levels were detected using quantitative reverse transcription polymerase chain reaction and Western blotting, respectively. Chemosensitivity to 5-fluorouracil (5-FU) was examined by cell proliferation assay and cell apoptosis assay. ECRG4 mRNA and protein expression levels were significantly upregulated in SGC-7901/ECRG4 cells induced with tetracycline. Compared with control cells, the growth inhibition rate of cells with ECRG4 overexpression was significantly increased when treated with 5-FU. Treatment with 5 μmol/l 5-FU resulted in 15.2 % apoptotic cells, whereas such treatment after overexpression of ECRG4 resulted in 44.5 % apoptotic cells. In conclusion, overexpression of ECRG4 enhanced the chemosensitivity of gastric cancer SGC-7901 cells to 5-FU through induction of apoptosis.

Keywords

ECRG4 Chemosensitivity 5-FU 

Notes

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Cheng-Ping Jiang
    • 1
  • Bi-Hua Wu
    • 1
  • Bai-Qiang Wang
    • 1
  • Mao-Yong Fu
    • 1
  • Ming Yang
    • 1
  • Yue Zhou
    • 1
  • Fu Liu
    • 1
  1. 1.Department of PharmacyAffiliated Hospital of North Sichuan Medical CollegeNanchongChina

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